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1. WO2012067572 - NEW SACCHAROMYCES CEREVISIAE STRAINS

Publication Number WO/2012/067572
Publication Date 24.05.2012
International Application No. PCT/SE2011/051369
International Filing Date 15.11.2011
IPC
C12N 15/01 2006.01
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
01Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
C12N 1/36 2006.01
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
36Adaptation or attenuation of cells
C12N 1/19 2006.01
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
14Fungi ; Culture media therefor
16Yeasts; Culture media therefor
19modified by introduction of foreign genetic material
C12P 7/10 2006.01
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7Preparation of oxygen-containing organic compounds
02containing a hydroxy group
04acyclic
06Ethanol, i.e. non-beverage
08produced as by-product or from waste or cellulosic material substrate
10substrate containing cellulosic material
C12R 1/865 2006.01
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RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C-C12Q75
1Microorganisms
645Fungi
85Saccharomyces
865Saccharomyces cerevisiae
CPC
C12N 15/01
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NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
01Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
C12N 15/81
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NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
80for fungi
81for yeasts
C12N 9/0006
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9Enzymes; Proenzymes; Compositions thereof
0004Oxidoreductases (1.)
0006acting on CH-OH groups as donors (1.1)
C12N 9/1205
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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9Enzymes; Proenzymes; Compositions thereof
10Transferases (2.)
12transferring phosphorus containing groups, e.g. kinases (2.7)
1205Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
C12P 7/06
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7Preparation of oxygen-containing organic compounds
02containing a hydroxy group
04acyclic
06Ethanol, i.e. non-beverage
C12P 7/10
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7Preparation of oxygen-containing organic compounds
02containing a hydroxy group
04acyclic
06Ethanol, i.e. non-beverage
08produced as by-product or from waste or cellulosic material substrate
10substrate containing cellulosic material
Applicants
  • SCANDINAVIAN TECHNOLOGY GROUP AB [SE]/[SE] (AllExceptUS)
  • ALBERS, Eva [SE]/[SE] (UsOnly)
  • OLSSON, Lisbeth [SE]/[SE] (UsOnly)
  • KOPPRAM, Rakesh [IN]/[SE] (UsOnly)
Inventors
  • ALBERS, Eva
  • OLSSON, Lisbeth
  • KOPPRAM, Rakesh
Agents
  • AWAPATENT AB
Priority Data
1051194-715.11.2010SE
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) NEW SACCHAROMYCES CEREVISIAE STRAINS
(FR) NOUVELLES SOUCHES DE SACCHAROMYCES CEREVISIAE
Abstract
(EN)
A method for producing astrain of Saccharomyces cerevisiae with introduced genes coding for xylose reductase, xylitol dehydrogenase and xylulokinase and with improved ethanol production, improved xylose conversion and reduced xylitol production is described. The method comprises culturing the cells in a repetitive batch series in a medium at a xylose concentration of about 15-25 g/l and at a temperature of about 28- 32°C, and thereafter lowering the xylose concentration in at least one step at a temperature of about 28-32°C to obtain an increased selection pressure for improved xylose fermentation,improved ethanol production and reduced xylitol production, and continuing the culturing of the cells in said repetitive batch series. Further, strains of Saccharomyces cerevisiae obtained by the method according to the invention are described.
(FR)
L'invention concerne un procédé de production d'une souche de Saccharomyces cerevisiae ayant des gènes introduits codant pour la xylose réductase, la xylitol déshydrogénase et la xylulokinase, et ayant une production d'éthanol améliorée, une conversion de xylose améliorée et une production de xylitol réduite. Le procédé comprend la mise en culture des cellules dans une série par lots répétés dans un milieu à une concentration de xylose d'environ 15-25 g/l et à une température d'environ 28-32°C, et ensuite la diminution de la concentration de xylose dans au moins une étape à une température d'environ 28-32°C pour obtenir une pression de sélection accrue pour une fermentation de xylose améliorée, une production d'éthanol améliorée et une production de xylitol réduite, et la poursuite de la culture des cellules selon ladite série par lots répétés. De plus, l'invention concerne des souches de Saccharomyces cerevisiae obtenues par le procédé selon l'invention.
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