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1. WO2012064619 - PICHIA PASTORIS LOCI ENCODING ENZYMES IN THE PROLINE BIOSYNTHETIC PATHWAY

Publication Number WO/2012/064619
Publication Date 18.05.2012
International Application No. PCT/US2011/059491
International Filing Date 07.11.2011
IPC
C12N 15/81 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
80for fungi
81for yeasts
CPC
C12N 15/815
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
80for fungi
81for yeasts
815for yeasts other than Saccharomyces
Applicants
  • MERCK SHARP & DOHME CORP. [US]/[US] (AllExceptUS)
  • NETT, Juergen [DE]/[US] (UsOnly)
Inventors
  • NETT, Juergen
Common Representative
  • MERCK SHARP & DOHME CORP.
Priority Data
61/411,95810.11.2010US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) PICHIA PASTORIS LOCI ENCODING ENZYMES IN THE PROLINE BIOSYNTHETIC PATHWAY
(FR) LOCUS DE PICHIA PASTORIS CODANT POUR DES ENZYMES DANS LA VOIE BIOSYNTHÉTIQUE DE LA PROLINE
Abstract
(EN)
Disclosed is the PRO2 or PRO3 gene of Pichia pastoris. The locus is useful sites for stable integration of heterologous nucleic acid molecules into the Pichia pastoris genome. The gene or gene fragments encoding the Pro2p or Pro3p may be used as a selection marker for constructing recombinant Pichia pastoris.
(FR)
L'invention divulgue le gène PRO2 ou PRO3 de Pichia pastoris. Le locus représente des sites utiles pour une intégration stable de molécules d'acide nucléique hétérologue dans le génome de Pichia pastoris. Le gène ou les fragments de gène codant pour la Pro2p ou la Pro3p peuvent être utilisés comme marqueur de sélection pour la construction de Pichia pastoris recombinant.
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