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1. WO2012063736 - GENETIC TESTING METHOD AND TESTING APPARATUS

Publication Number WO/2012/063736
Publication Date 18.05.2012
International Application No. PCT/JP2011/075441
International Filing Date 04.11.2011
IPC
C12Q 1/68 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
C12M 1/00 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
G01N 35/00 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
35Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/-G01N33/148; Handling materials therefor
C12N 15/00 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
CPC
B01L 7/52
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
7Heating or cooling apparatus
52with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
C12Q 1/686
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6844Nucleic acid amplification reactions
686Polymerase chain reaction [PCR]
G01N 2035/00346
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
35Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
00346Heating or cooling arrangements
G01N 2035/00366
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
35Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
00346Heating or cooling arrangements
00356Holding samples at elevated temperature (incubation)
00366Several different temperatures used
G01N 2035/009
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
35Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
00584Control arrangements for automatic analysers
00722Communications; Identification
00891Displaying information to the operator
009alarms, e.g. audible
G01N 35/00623
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
35Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
00584Control arrangements for automatic analysers
00594Quality control, including calibration or testing of components of the analyser
00613Quality control
00623of instruments
Applicants
  • 株式会社日立ハイテクノロジーズ HITACHI HIGH-TECHNOLOGIES CORPORATION [JP]/[JP] (AllExceptUS)
  • 石沢 雅人 ISHIZAWA Masato [JP]/[JP] (UsOnly)
  • 庄司 義之 SHOJI Yoshiyuki [JP]/[JP] (UsOnly)
  • 佐野 稔 SANO Minoru [JP]/[JP] (UsOnly)
Inventors
  • 石沢 雅人 ISHIZAWA Masato
  • 庄司 義之 SHOJI Yoshiyuki
  • 佐野 稔 SANO Minoru
Agents
  • ポレール特許業務法人 POLAIRE I.P.C.
Priority Data
2010-25157610.11.2010JP
Publication Language Japanese (JA)
Filing Language Japanese (JA)
Designated States
Title
(EN) GENETIC TESTING METHOD AND TESTING APPARATUS
(FR) MÉTHODE DE MISE EN ŒUVRE DE TESTS GÉNÉTIQUES ET APPAREIL AFFÉRENT
(JA) 遺伝子検査方法及び検査装置
Abstract
(EN)
Provided are highly reliable genetic testing method and testing apparatus, with which, when a temperature control device is provided for each temperature control block in which a reaction vessel is installed so that a plurality of reaction solutions requiring different protocols can be simultaneously treated and even if an analytical treatment is being performed, a treatment of another reaction solution can be started, an individual difference among the temperature control devices due to factors possibly occurring in industrial products is eliminated, and an abnormal operation associated with the temperature control mechanism of a reaction vessel for amplifying a target nucleic acid can be easily detected. The genetic testing method characterized in that the temperatures of a plurality of reaction vessels, each of which stores an amplification solution containing a target nucleic acid to be amplified and components necessary for amplification, are independently controlled by a thermostat bath, a temperature-measuring element, and a heating/cooling element provided for each reaction vessel, and according to an analysis/testing protocol previously determined for each position of the reaction vessel, the temperature is controlled for each installed position of the reaction vessel and each predetermined temperature set for the position, and the value of the controlled temperature of the reaction vessel is output on a monitor, the value of the controlled temperature is monitored for each reaction vessel and the correction value of the controlled temperature is calculated and stored for each reaction vessel, the temperature of the reaction vessel is controlled on the basis of the calculated value, and light emitted from the amplification solution stored in the reaction vessel is measured, and the apparatus therefor.
(FR)
Cette invention concerne une méthode de mise en œuvre de tests génétiques et un appareil afférent très fiables, permettant, quand un dispositif de régulation de température est utilisé pour chaque bloc de régulation de température dans lequel un réacteur est installé de façon qu'une pluralité de solutions réactionnelles nécessitant des protocoles différents puisse être traitée simultanément, et que le traitement d'une autre solution réactionnelle puisse être démarré, même si un traitement analytique est en cours, d'éliminer une différence individuelle au sein desdits dispositifs de régulation de température due à des facteurs pouvant survenir dans des produits industriels et de détecter facilement tout fonctionnement anormal associé au mécanisme de régulation de température d'un réacteur destiné à amplifier un acide nucléique cible. La méthode de mise en œuvre de tests génétiques selon l'invention est caractérisée en ce que les températures d'une pluralité de réacteurs, contenant chacun une solution d'amplification contenant un acide nucléique cible à amplifier et les composants nécessaires à l'amplification, sont indépendamment régulées par un bain thermostatique, un élément de mesure de la température, et un élément de chauffage/refroidissement équipant chacun desdits réacteurs, et en fonction d'un protocole d'analyse/test préalablement déterminé pour chaque position du réacteur. La température est régulée pour chaque position installée du réacteur et chaque température prédéterminée fixée pour la position, et la valeur de la température régulée du réacteur s'affiche sur un moniteur, la valeur de la température régulée est surveillée pour chaque réacteur et la valeur de correction de la température régulée est calculée et enregistrée pour chaque réacteur, la température du réacteur étant régulée en fonction de la valeur calculée, et la lumière émise par la solution d'amplification contenue dans le réacteur est mesurée. L'appareil permettant la mise en œuvre de la méthode selon l'invention est également décrit.
(JA)
プロトコルの異なる複数種類の反応液を同時に処理でき、且つ、実行中の分析処理があっても別反応液の処理を開始できるようにするために、反応容器を架設する温調ブロックごとにそれぞれ温度制御装置を設けるが、その際、工業製品に生じうる要因による複数の温度制御装置の個体差をなくし、さらに、標的核酸増幅の反応容器の温度制御機構に係る異常動作を容易に認識できる高信頼性の遺伝子検出方法及び検出装置を提供する。増幅対象の標的核酸及び増幅に必要な成分を含む増幅液を収容する複数個の反応容器のそれぞれに付設された恒温槽及び測温素子と加熱・冷却素子によって、前記反応容器のそれぞれを独立に温度制御し、前記反応容器のポジション毎に予め決められた分析・検査プロトコルに従って、前記反応容器の架設ポジションとそのポジションに設定された所定の温度ごとに温度制御を行い、かつ前記反応容器の制御温度の値をモニターに出力し、前記反応容器単位で制御温度の値の監視及び反応容器単位で制御温度の補正値を演算・格納し、その演算値に基づいて前記反応容器の温度制御を実行し、前記反応容器内に収容する増幅液の発光を測定することを特徴とする遺伝子検査方法及びその装置。
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