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1. WO2012063084 - BACTERIAL METHODS

Publication Number WO/2012/063084
Publication Date 18.05.2012
International Application No. PCT/GB2011/052210
International Filing Date 11.11.2011
Chapter 2 Demand Filed 11.09.2012
IPC
C12N 3/00 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
3Spore-forming or isolating processes
C07K 14/32 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
195from bacteria
32from Bacillus (G)
C07K 14/33 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
195from bacteria
33from Clostridium (G)
CPC
C07K 14/32
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
195from bacteria
32from Bacillus (G)
C12N 15/75
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
75for Bacillus
C12N 3/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
3Spore forming or isolating processes
C12N 9/12
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
10Transferases (2.)
12transferring phosphorus containing groups, e.g. kinases (2.7)
Applicants
  • IMPERIAL INNOVATIONS LIMITED [GB]/[GB] (AllExceptUS)
  • BAYER, Travis Scott [US]/[GB] (UsOnly)
  • SAMSON, Jennifer, Adele [GB]/[GB] (UsOnly)
Inventors
  • BAYER, Travis Scott
  • SAMSON, Jennifer, Adele
Agents
  • PILKINGTON, Stephanie, Joan
Priority Data
1019086.611.11.2010GB
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) BACTERIAL METHODS
(FR) PROCÉDÉS BACTÉRIENS
Abstract
(EN)
A bacterial spore comprising a modified prkC protein, wherein the extracellular domain of the modified prkC protein binds an agent which is not bound by the extracellular domain of the wild- type prkC protein, and wherein the agent is a germinant that stimulates germination of the bacterial spore, or a bacterial spore comprising a modified gerA protein, wherein the gerA protein has been modified such that the spore undergoes germination in the presence of a germinant which does not stimulate germination of a bacterial spore comprising wild- type gerA protein.
(FR)
La présente invention concerne une spore bactérienne comprenant une protéine prkC modifiée. Le domaine extracellulaire de la protéine prkC modifiée se lie à un agent auquel ne se lie pas le domaine extracellulaire de la protéine prkC de type sauvage. Cet agent, qui est un germinat stimulant la germination de la spore bactérienne, peut également être une spore bactérienne comprenant la protéine prkC modifiée. Enfin, la protéine gerA a été modifiée de façon que la spore subisse la germination en présence d'un germinat qui ne stimule pas la germination d'une spore bactérienne comprenant la protéine gerA de type sauvage.
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