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1. WO2012062943 - LIPASE LIPBL AND THE APPLICATIONS THEREOF

Publication Number WO/2012/062943
Publication Date 18.05.2012
International Application No. PCT/ES2011/000325
International Filing Date 08.11.2011
IPC
C12N 9/16 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14Hydrolases (3.)
16acting on ester bonds (3.1)
C12P 19/00 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
C12N 11/02 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
11Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
02Enzymes or microbial cells immobilised on or in an organic carrier
C07H 13/02 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
13Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids
02by carboxylic acids
CPC
C12N 9/20
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
14Hydrolases (3)
16acting on ester bonds (3.1)
18Carboxylic ester hydrolases ; (3.1.1)
20Triglyceride splitting, e.g. by means of lipase
C12P 13/008
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
13Preparation of nitrogen-containing organic compounds
008containing a N-O bond, e.g. nitro (-NO2), nitroso (-NO)
C12P 19/12
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
12Disaccharides
C12P 41/003
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
41Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture
003by ester formation, lactone formation or the inverse reactions
C12P 7/42
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7Preparation of oxygen-containing organic compounds
40containing a carboxyl group ; including Peroxycarboxylic acids
42Hydroxy-carboxylic acids
C12P 7/62
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7Preparation of oxygen-containing organic compounds
62Carboxylic acid esters
Applicants
  • UNIVERSIDAD DE SEVILLA [ES]/[ES] (AllExceptUS)
  • CONSEJO SUPERIOR DE INVESTIGACIONES CIENTÍFICAS [ES]/[ES] (AllExceptUS)
  • PÉREZ GÓMEZ, Dolores [ES]/[ES] (UsOnly)
  • VENTOSA UCERO, Antonio [ES]/[ES] (UsOnly)
  • MELLADO DURÁN, Encarnación [ES]/[ES] (UsOnly)
  • GUISAN SEIJAS, José Manuel [ES]/[ES] (UsOnly)
  • FERNÁNDEZ LORENTE, Gloria [ES]/[ES] (UsOnly)
  • FILICE, Marco [IT]/[IT] (UsOnly)
Inventors
  • PÉREZ GÓMEZ, Dolores
  • VENTOSA UCERO, Antonio
  • MELLADO DURÁN, Encarnación
  • GUISAN SEIJAS, José Manuel
  • FERNÁNDEZ LORENTE, Gloria
  • FILICE, Marco
Priority Data
P20103163608.11.2010ES
Publication Language Spanish (ES)
Filing Language Spanish (ES)
Designated States
Title
(EN) LIPASE LIPBL AND THE APPLICATIONS THEREOF
(ES) LIPASA LIPBL Y SUS APLICACIONES
(FR) LIPASE LIPBL ET SES APPLICATIONS
Abstract
(EN)
The invention relates to the use of the lipase LipBL for the hydrolysis of different substrates selected from p-nitrophenols, tributyrin, Tween 80, oils, chiral and prochiral substrates, fish oil, peracetylated sugars or nitrocefin, and to an enzymatic method for the regioselective monodeprotection of peracetylated sugars for producing monodeacetylated sugars.
(ES)
La presente invención se refiere al uso de la lipasa LipBL para la hidrólisis de distintos sustratos seleccionados de entre p-nitrofenoles, tributirina, Tween 80, aceites, sustratos quirales y proquirales, aceite de pescado, azúcares paracetilados o nitrocefin y a un procedimiento enzimático de monodesprotección regioselectiva de azúcares paracetilados para la obtención de azúcares monodesacteilados.
(FR)
La présente invention concerne l'utilisation de la lipase LipBL pour l'hydrolyse de différents substrats sélectionnés parmi les p-nitrophénols, la tributirine, le Tween 80, les huiles, les substrats chiraux et prochiraux, l'huile de poisson, les glucides paracétylés ou la nitrocéphine, ainsi qu'un procédé enzymatique de monodéprotection régiosélective de glucides paracétylés pour l'obtention de glucides monodésacétylés.
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