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1. WO2012061405 - DIFFERENTIAL DETECTION AND QUANTIFICATION OF OXALOBACTER

Publication Number WO/2012/061405
Publication Date 10.05.2012
International Application No. PCT/US2011/058808
International Filing Date 01.11.2011
IPC
C12N 15/11 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
C12Q 1/68 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
G01N 33/569 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
569for microorganisms, e.g. protozoa, bacteria, viruses
G01N 33/52 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
52Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper
CPC
C12Q 1/689
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
6888for detection or identification of organisms
689for bacteria
C12Q 2600/156
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
2600Oligonucleotides characterized by their use
156Polymorphic or mutational markers
Applicants
  • UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC. [US]/[US] (AllExceptUS)
  • NGUYEN, Cuong, Quoc [US]/[US] (UsOnly)
  • PECK, Ammon, B. [US]/[US] (UsOnly)
Inventors
  • NGUYEN, Cuong, Quoc
  • PECK, Ammon, B.
Agents
  • HUNTER-ENSOR, Melissa
Priority Data
61/408,70601.11.2010US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) DIFFERENTIAL DETECTION AND QUANTIFICATION OF OXALOBACTER
(FR) DÉTECTION ET QUANTIFICATION DIFFÉRENTIELLES D'OXALOBACTER
Abstract
(EN)
Disclosed herein are PNA probes, or PNA probe sets and their use as well as kits useful for the analysis of certain Oxalobacter species and/or strain(s) present in a sample of interest. Probe sequences may sequence that are at least about 86% identical to the nucleobase sequence or complement thereof selected from the following sequences: GACAATGTAGAGTTGACT (SED ID NO. 1 ); caggatggtcagaagttc (SEQ ID NO. 2); CCGGTTACATCGAAGGA (SEQ ID NO. 3); and AATGTAGAGTTG ACT (SEQ ID NO. 4).
(FR)
La présente invention concerne des sondes d'APN, ou des ensembles de sondes de APN et leur utilisation ainsi que des kits utiles pour l'analyse de certaines espèces et/ou souche(s) d'Oxalobacter présentes dans un échantillon examiné. Les séquences de sonde peuvent avoir une séquence qui est au moins environ 86 % identique à la séquence de nucléobases ou la séquence complémentaire de celle-ci choisie parmi les séquences suivantes : GACAATGTAGAGTTGACT (SED ID NO: 1); caggatggtcagaagttc (SED ID NO: 2) ; CCGGTTACATCGAAGGA (SED ID NO: 3) ; et AATGTAGAGTTGACT (SED ID NO: 4).
Also published as
Latest bibliographic data on file with the International Bureau