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1. WO2012061105 - HOMOZYGOUS AND HETEROZYGOUS IDH1 GENE-DEFECTIVE CELL LINES DERIVED FROM HUMAN COLORECTAL CELLS

Publication Number WO/2012/061105
Publication Date 10.05.2012
International Application No. PCT/US2011/057615
International Filing Date 25.10.2011
IPC
C12N 5/10 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
10Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
C12N 5/09 2010.01
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
09Tumour cells
C12N 15/85 2006.01
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
85for animal cells
A01K 67/027 2006.01
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
67Rearing or breeding animals, not otherwise provided for; New breeds of animals
027New breeds of vertebrates
CPC
C12N 15/85
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
85for animal cells
C12N 15/902
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
90Stable introduction of foreign DNA into chromosome
902using homologous recombination
C12N 2510/00
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2510Genetically modified cells
C12N 2799/025
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2799Uses of viruses
02as vector
021for the expression of a heterologous nucleic acid
025where the vector is derived from a parvovirus
C12N 2800/30
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2800Nucleic acids vectors
30Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
C12N 5/0693
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor;
06Animal cells or tissues; Human cells or tissues
0602Vertebrate cells
0693Tumour cells; Cancer cells
Applicants
  • DUKE UNIVERSITY [US]/[US] (AllExceptUS)
  • YAN, Hai [US]/[US] (UsOnly)
  • BIGNER, Darell [US]/[US] (UsOnly)
  • DUNCAN, Christopher Gentry [US]/[US] (UsOnly)
Inventors
  • YAN, Hai
  • BIGNER, Darell
  • DUNCAN, Christopher Gentry
Agents
  • KAGAN, Sarah
Priority Data
61/406,26825.10.2010US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) HOMOZYGOUS AND HETEROZYGOUS IDH1 GENE-DEFECTIVE CELL LINES DERIVED FROM HUMAN COLORECTAL CELLS
(FR) LIGNÉES CELLULAIRES DÉFICIENTES EN GÈNE IDH1 HOMOZYGOTES ET HÉTÉROZYGOTES DÉRIVÉES DE CELLULES COLORECTALES HUMAINES
Abstract
(EN)
IDH1 gene-defective cell lines (e.g., IDH1R132H heterozygous) have been made from a robust cell line, HCT116. The IDH1 gene-defective cell lines can be used to determine the effect of IDH1R132H on cell biology, tumorigenesis, and cellular metabolic profiles. These cell lines can be used to test potential therapeutic targets and to screen potential therapeutic agents. Kits and xenografts are also contemplated.
(FR)
La présente invention concerne des lignées cellulaires déficientes en gène IDH1 (par exemple, IDH1R132H hétérozygote) qui ont été produites à partir d'une lignée cellulaire robuste, HCT116. Les lignées cellulaires déficientes en gène IDH1 peuvent être utilisées pour déterminer l'effet de IDH1R132H sur la biologie cellulaire, la cancérogenèse, et des profils métaboliques cellulaires. Ces lignées cellulaires peuvent être utilisées pour soumettre à essai des cibles thérapeutiques potentielles et cribler des agents thérapeutiques potentiels. La présente invention concerne en outre des kits et des xénogreffes.
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