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1. WO2012059784 - METHOD AND DEVICE FOR FLUORESCENT MEASUREMENT OF SAMPLES

Publication Number WO/2012/059784
Publication Date 10.05.2012
International Application No. PCT/IB2010/054965
International Filing Date 03.11.2010
IPC
G01N 21/64 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63optically excited
64Fluorescence; Phosphorescence
G02B 21/00 2006.01
GPHYSICS
02OPTICS
BOPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
21Microscopes
CPC
G01N 21/6428
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63optically excited
64Fluorescence; Phosphorescence
6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
G01N 21/6452
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63optically excited
64Fluorescence; Phosphorescence
645Specially adapted constructive features of fluorimeters
6452Individual samples arranged in a regular 2D-array, e.g. multiwell plates
G02B 21/0076
GPHYSICS
02OPTICS
BOPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
21Microscopes
0004specially adapted for specific applications
002Scanning microscopes
0024Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
0052Optical details of the image generation
0076arrangements using fluorescence or luminescence
Applicants
  • REAMETRIX INC [US]/[US] (AllExceptUS)
  • MANIAN, Bala S [US]/[US] (UsOnly)
Inventors
  • MANIAN, Bala S
Priority Data
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHOD AND DEVICE FOR FLUORESCENT MEASUREMENT OF SAMPLES
(FR) PROCÉDÉ ET DISPOSITIF DE MESURE DE FLUORESCENCE D'ÉCHANTILLONS
Abstract
(EN)
The invention provides a method of fluorescent measurement of a sample and a corresponding measuring device. The method includes impinging an incident beam having an excitation wavelength and a focus diameter on the sample to yield a laser spot that defines a sample volume for measuring fluorescence. The method then includes displacing the laser spot relative to the sample volume in a three dimensional space defined by the sample volume to define an individual volume of interest. The method further comprises translating the laser spot across the sample volume in a depth direction for the purpose of defining a depth profile based on a thickness of the sample carrier by detecting one or more emitted fluorescence signals from the sample volume in the depth direction, and further determining at least one microvolume of interest from the depth profile for obtaining at least one concentrated emitted fluorescence signal.
(FR)
L'invention concerne un procédé de mesure de fluorescence d'un échantillon et un dispositif de mesure correspondant. Le procédé consiste à projeter un faisceau incident ayant une longueur d'onde d'excitation et un diamètre de foyer sur l'échantillon afin d'obtenir un point laser qui définit un volume d'échantillon permettant de mesurer la fluorescence. Le procédé consiste ensuite à déplacer le point laser par rapport au volume de l'échantillon pour définir un volume individuel à étudier. Le procédé consiste en outre à translater le point laser dans tout le volume échantillon dans la direction de la profondeur afin de définir un profil de profondeur fondé sur une épaisseur du porte-échantillon en détectant un ou plusieurs signaux de fluorescence émis provenant du volume échantillon dans la direction de la profondeur, et pour déterminer en outre au moins un micro-volume considéré à partir du profil de profondeur de manière à obtenir au moins un signal de fluorescence concentré émis.
Also published as
Latest bibliographic data on file with the International Bureau