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1. WO2012055971 - MICROVESICLES DERIVED FROM ATHEROPROTECTIVE ENDOTHELIAL CELLS FOR THE TREATMENT AND PREVENTION OF ATHEROSCLEROTIC DISEASES

Publication Number WO/2012/055971
Publication Date 03.05.2012
International Application No. PCT/EP2011/068877
International Filing Date 27.10.2011
IPC
A61P 9/10 2006.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
9Drugs for disorders of the cardiovascular system
10for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
A61K 48/00 2006.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
48Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
A61K 9/51 2006.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
9Medicinal preparations characterised by special physical form
48Preparations in capsules, e.g. of gelatin, of chocolate
50Microcapsules
51Nanocapsules
CPC
A61K 9/127
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
9Medicinal preparations characterised by special physical form
10Dispersions; Emulsions
127Liposomes
A61K 9/5176
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
9Medicinal preparations characterised by special physical form
48Preparations in capsules, e.g. of gelatin, of chocolate
50Microcapsules ; having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
51Nanocapsules; Nanoparticles;
5107Excipients; Inactive ingredients
5176Compounds of unknown constitution, e.g. material from plants or animals
A61P 9/10
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
9Drugs for disorders of the cardiovascular system
10for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
C12N 15/111
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
111General methods applicable to biologically active non-coding nucleic acids
C12N 2310/141
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2310Structure or type of the nucleic acid
10Type of nucleic acid
14interfering N.A.
141MicroRNAs, miRNAs
C12N 2320/32
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2320Applications; Uses
30Special therapeutic applications
32Special delivery means, e.g. tissue-specific
Applicants
  • JOHANN WOLFGANG GOETHE-UNIVERSITÄT, FRANKFURT AM MAIN [DE]/[DE] (AllExceptUS)
  • DIMMELER, Stefanie [DE]/[DE] (UsOnly)
  • BOON, Reinier [NL]/[DE] (UsOnly)
  • HERGENREIDER, Eduard [DE]/[DE] (UsOnly)
Inventors
  • DIMMELER, Stefanie
  • BOON, Reinier
  • HERGENREIDER, Eduard
Agents
  • KRAUSS, Jan B.
Priority Data
10189093.727.10.2010EP
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) MICROVESICLES DERIVED FROM ATHEROPROTECTIVE ENDOTHELIAL CELLS FOR THE TREATMENT AND PREVENTION OF ATHEROSCLEROTIC DISEASES
(FR) MICROVÉSICULES ISSUES DE CELLULES ENDOTHÉLIALES ATHÉROPROTECTRICES POUR LE TRAITEMENT ET LA PRÉVENTION DE MALADIES ATHÉROSCLÉROTIQUES
Abstract
(EN)
The present invention relates to methods for the production of atheroprotective vesicles. The invention is based on the finding that endothelial cells wherein the activity of the transcription factor KLF2 is induced and/or enhanced surprisingly produce and/or secrete vesicles enclosing various atheroprotective microRNAs, in particular microRNAs of the microRNA-cluster miR-143/145. Thus, the present invention discloses methods for the production of vesicles derived from atheroprotective endothelial cells and the vesicles produced by the disclosed methods. Furthermore artificially produced vesicles enclosing the atheroprotective microRNA composition according to the invention are provided. The vesicles of the present invention are useful in the treatment and prevention of atherosclerotic diseases, such as acute coronary syndromes, plaque rupture, aneurysms, in-stent restenosis, ischemic diseases and heart failure.
(FR)
La présente invention concerne des procédés de production de vésicules athéroprotectrices. L'invention se base sur la découverte que les cellules endothéliales dans lesquelles l'activité du facteur de transcription KLF2 est induite et/ou accrue produisent et/ou secrètent de manière surprenante des vésicules comprenant divers microARN athéroprotecteurs, en particulier des microARN du groupe de microARN miR-143/145. Ainsi, la présente invention concerne des procédés de production de vésicules issues de cellules endothéliales athéroprotectrices, et les vésicules produites par les procédés décrits. L'invention concerne en outre des vésicules produites artificiellement, comprenant la composition de microARN athéroprotectrice selon l'invention. Les vésicules de la présente invention sont utiles dans le traitement et la prévention de maladies athérosclérotiques, telles que des syndromes coronariens aigus, la rupture d'une plaque, des anévrismes, une resténose intrastent, des maladies ischémiques et une insuffisance cardiaque.
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