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1. (WO2012054727) REVERSE TRANSCRIPTASE MIXTURES WITH IMPROVED STORAGE STABILITY
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2012/054727    International Application No.:    PCT/US2011/057103
Publication Date: 26.04.2012 International Filing Date: 20.10.2011
IPC:
C12P 19/34 (2006.01)
Applicants: BIO-RAD LABORATORIES, INC. [US/US]; 1000 Alfred Nobel Drive Hercules, California 94547 (US) (For All Designated States Except US).
MA, Jason [US/US]; (US) (For US Only).
GONG, Xiao-Song [CN/US]; (US) (For US Only)
Inventors: MA, Jason; (US).
GONG, Xiao-Song; (US)
Agent: HINSCH, Matthew E.; Kilpatrick Townsend & Stockton LLP Two Embarcadero Center, Eighth Floor San Francisco, CA 94111-3834 (US)
Priority Data:
61/405,961 22.10.2010 US
Title (EN) REVERSE TRANSCRIPTASE MIXTURES WITH IMPROVED STORAGE STABILITY
(FR) MÉLANGES DE TRANSCRIPTASE INVERSE PRÉSENTANT UNE STABILITÉ DE STOCKAGE AMÉLIORÉE
Abstract: front page image
(EN)Reverse transcriptase mixtures with improved storage stability are provided. In one embodiment, a reaction mixture is disclosed comprising sufficient ingredients, except template RNA, for reverse transcription of RNA, the mixture comprising: a reverse transcriptase having an optimal pH above 8; and a buffer, wherein the mixture has a pH of between 6-8, lacks template RNA, and the reverse transcriptase maintains essentially all reverse transcriptase activity following incubation of the mixture at 50° C for 30 minutes.
(FR)L'invention concerne des mélanges de transcriptase inverse présentant une stabilité de stockage améliorée. Dans un mode de réalisation, un mélange réactionnel est décrit, qui comprend des ingrédients suffisants, à l'exclusion d'un ARN messager, pour accomplir la transcription inverse de l'ARN. Le mélange comprend une transcriptase inverse à pH optimal supérieur à 8, et un tampon. Le mélange présente un pH compris entre 6 et 8, est exempt d'ARN messager, et la transcriptase inverse retient sensiblement toute l'activité transcriptase inverse après incubation du mélange à 50°C pendant 30 minutes.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)