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Machine translation
1. (WO2012052711) METHOD FOR IDENTIFYING ACTIVATION OF TRANSFERASES
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2012/052711    International Application No.:    PCT/GB2011/001497
Publication Date: 26.04.2012 International Filing Date: 18.10.2011
IPC:
C12Q 1/48 (2006.01), G01N 33/68 (2006.01)
Applicants: QUEEN MARY & WESTFIELD COLLEGE, UNIVERSITY OF LONDON [GB/GB]; Mile End Road London E1 4NS (GB) (For All Designated States Except US).
RODRIGUEZ CUTILLAS, Pedro [ES/GB]; (GB) (For US Only).
VANHAESEBROECK, Bart [BE/GB]; (GB) (For US Only).
BELTRAN, Luisa, Marie [GB/GB]; (GB) (For US Only)
Inventors: RODRIGUEZ CUTILLAS, Pedro; (GB).
VANHAESEBROECK, Bart; (GB).
BELTRAN, Luisa, Marie; (GB)
Agent: LAU, Sarah, Jane; Kilburn & Strode LLP 20 Red Lion Street London WC1R 4PJ (GB)
Priority Data:
1017721.0 20.10.2010 GB
Title (EN) METHOD FOR IDENTIFYING ACTIVATION OF TRANSFERASES
(FR) PROCÉDÉ D'IDENTIFICATION D'ACTIVATION DE TRANSFÉRASES
Abstract: front page image
(EN)The present invention provides a method for identifying differential activation of a bisubstrate protein modifying enzyme between samples, comprising: (i) incubating a first sample with x different concentrations of the non-protein substrate of said enzyme, wherein x is 2 or greater than 2; (ii) quantifying modification of a polypeptide in said sample at each of the x different concentrations of the non-protein substrate; (iii) determining the affinity of said enzyme for said non-protein substrate; (iv) repeating steps (i) to (iii) for a second or subsequent sample; and (v) comparing the affinity of said enzyme for said non-protein substrate between said samples; wherein a difference in affinity of said enzyme for said non-protein substrate between samples is indicative of differential activation of said enzyme between samples. The present invention also provides a method for identifying an in vivo substrate of a bisubstrate protein modifying enzyme.
(FR)Cette invention concerne un procédé d'identification de l'activation différentielle d'une enzyme modifiant une protéine bisubstrat entre des échantillons, ledit procédé comprenant : (i) l'incubation d'un premier échantillon à x concentrations différentes du substrat non protéique de ladite enzyme, x valant 2 ou plus; (ii) la quantification de la modification d'un polypeptide dans ledit échantillon à chacune des x concentrations différentes du substrat non protéique; (iii) la détermination de l'affinité de ladite enzyme pour ledit substrat non protéique; (iv) la répétition des étapes (i) à (iii) pour un deuxième échantillon ou un échantillon suivant; et (v) la comparaison de l'affinité de ladite enzyme pour ledit substrat non protéique entre lesdits échantillons, toute différence d'affinité de ladite enzyme pour ledit substrat non protéique entre les échantillons indiquant une activation différentielle de ladite enzyme entre les échantillons. Cette invention concerne également un procédé permettant d'identification d'un substrat in vivo d'une enzyme modifiant une protéine bisubstrat.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)