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1. (WO2012050981) DEVICE FOR HIGH THROUGHPUT INVESTIGATIONS OF CELLULAR INTERACTIONS
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2012/050981    International Application No.:    PCT/US2011/054029
Publication Date: 19.04.2012 International Filing Date: 29.09.2011
IPC:
G01N 33/48 (2006.01), C12M 3/00 (2006.01), G01N 35/00 (2006.01), B01L 3/00 (2006.01), C12N 5/00 (2006.01), G03F 7/00 (2006.01)
Applicants: MASSACHUSETTS INSTITUTE OF TECHNOLOGY [US/US]; 77 Massachusetts Avenue Cambridge, MA 02139-4307 (US) (For All Designated States Except US).
KAMM, Roger, Dale [US/US]; (US) (For US Only).
ASADA, Haruhiko, Harry [US/US]; (US) (For US Only).
FARAHAT, Waleed, Ahmed [EG/US]; (US) (For US Only).
ZERVANTONAKIS, Loannis, K. [GR/US]; (US) (For US Only).
WOOD, Levi, B. [US/US]; (US) (For US Only).
KOTHAPALLI, Chandrasekhar [US/US]; (US) (For US Only).
CHUNG, Seok [KR/KR]; (KR) (For US Only).
MACKLIS, Jeffrey [--/US]; (US) (For US Only).
THARIN, Suzanne [--/US]; (US) (For US Only).
VARNER, Johanna [US/US]; (US) (For US Only).
PARK, Young, Kum [KR/SG]; (SG) (For US Only).
LEE, Kwang, Ho [--/US]; (US) (For US Only).
TU, Nguyen Le Thanh [VN/CH]; (CH) (For US Only).
KIM, Choong [--/US]; (US) (For US Only)
Inventors: KAMM, Roger, Dale; (US).
ASADA, Haruhiko, Harry; (US).
FARAHAT, Waleed, Ahmed; (US).
ZERVANTONAKIS, Loannis, K.; (US).
WOOD, Levi, B.; (US).
KOTHAPALLI, Chandrasekhar; (US).
CHUNG, Seok; (KR).
MACKLIS, Jeffrey; (US).
THARIN, Suzanne; (US).
VARNER, Johanna; (US).
PARK, Young, Kum; (SG).
LEE, Kwang, Ho; (US).
TU, Nguyen Le Thanh; (CH).
KIM, Choong; (US)
Agent: COLLINS, Anne, J.; Hamilton, Brook, Smith & Reynolds, P.C. 530 Virginia Rd. P.O. Box 9133 Concord, MA 01742-9133 (US)
Priority Data:
61/387,674 29.09.2010 US
61/394,624 19.10.2010 US
Title (EN) DEVICE FOR HIGH THROUGHPUT INVESTIGATIONS OF CELLULAR INTERACTIONS
(FR) DISPOSITIF D'ANALYSE À HAUT DÉBIT D'INTERACTIONS CELLULAIRES
Abstract: front page image
(EN)Provided herein are microfluidic devices that can be used as a 3D bioassay, e.g., for drug screening, personalized medicine, tissue engineering, wound healing, and other applications. The device has a series of channels {e.g., small fluid channels) in a small polymer block wherein one or more of the channels can be filled with a biologically relevant gel, such as collagen, which is held in place by posts. As shown herein, when the device is plated with cells such as endothelial cells, new blood vessels grow in the gel, which is thick enough for the cells to grow in three dimensions. Other channels, e.g., fluid channels, allow drugs or biological material to be exposed to the 3D cell growth. Cells, such as endothelial cells, can be cultured and observed as they grow on the surface of a 3D gel scaffold, where e.g., rates of angiogenesis can be measured, as well as intervascularization and extravascularization of cancerous cells.
(FR)L'invention concerne des dispositifs microfluidiques qui peuvent être utilisés comme dosage biologique 3D, par ex. pour le criblage de médicaments, la médecine personnalisée, l'ingénierie tissulaire, la cicatrisation, et d'autres applications. Le dispositif comporte une série de canaux (par ex., de petits canaux fluidiques) dans un petit bloc polymère, un ou plusieurs des canaux pouvant être remplis avec un gel biologique tel que le collagène qui est maintenu en place par des montants. Quand le dispositif est recouvert de cellules telles que des cellules endothéliales, de nouveaux vaisseaux sanguins se forment dans le gel, qui est suffisamment épais pour que les cellules se multiplient dans les trois dimensions. D'autres canaux, par ex. des canaux fluidiques, permettent d'exposer des médicaments ou de la matière biologique à une croissance cellulaire tridimensionnelle. Des cellules telles que des cellules endothéliales peuvent être cultivées et observées lorsqu'elles croissent sur la surface d'un échafaudage de gel 3D, où par ex. des taux d'angiogenèse peuvent être mesurés, de même que l'intervascularisation et l'extravascularisation de cellules cancéreuses.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)