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1. (WO2012048856) PROINSULIN WITH HELPER SEQUENCE
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2012/048856    International Application No.:    PCT/EP2011/005090
Publication Date: 19.04.2012 International Filing Date: 11.10.2011
IPC:
C07K 14/62 (2006.01), C12N 15/62 (2006.01)
Applicants: GLUCOMETRIX PVS GMBH [DE/DE]; Am Mühlenberg 11 14476 Potsdam (DE) (For All Designated States Except US).
PONOMARENKO, Sophia [DE/DE]; (DE) (For US Only).
HERMANN, Stefan [DE/DE]; (DE) (For US Only).
ECKERT, Kelvin [DE/DE]; (DE) (For US Only)
Inventors: PONOMARENKO, Sophia; (DE).
HERMANN, Stefan; (DE).
ECKERT, Kelvin; (DE)
Agent: BRITTINGER, Matthias; TER MEER STEINMEISTER & PARTNER GBR Mauerkircherstraße 45 81679 München (DE)
Priority Data:
10013554.0 12.10.2010 EP
Title (EN) PROINSULIN WITH HELPER SEQUENCE
(FR) PROINSULINE AVEC SÉQUENCE AUXILIAIRE
Abstract: front page image
(EN)The invention provides with a polypeptide comprising a proinsulin precursor and a helper sequence. The helper sequence enhances the expression of the proinsulin precursor in a recombinant organism and increases the yields of insulin after purification and processing of the insulin precursor. The invention is thus further concerned with a method of producing insulin, in which proinsulin is expressed as recombinant polypeptide in an organism such as E. coli, isolated from the organism and then processed to yield active insulin.
(FR)La présente invention concerne un polypeptide comprenant un précurseur de la proinsuline et une séquence auxiliaire. La séquence auxiliaire améliore l'expression du précurseur de la proinsuline dans un organisme recombinant et accroît les rendements d'insuline après la purification et le traitement du précurseur de l'insuline. L'invention concerne en outre un procédé de production d'insuline, dans lequel la proinsuline est exprimée sous la forme d'un polypeptide recombinant dans un organisme tel qu'E. coli, isolée de l'organisme puis transformée pour donner de l'insuline active.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)