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1. WO2012045756 - PROCESS FOR PRODUCING THREE-DIMENSIONAL TISSUE

Publication Number WO/2012/045756
Publication Date 12.04.2012
International Application No. PCT/EP2011/067344
International Filing Date 04.10.2011
IPC
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21
Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible, or ultra-violet light
62
Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63
optically excited
64
Fluorescence; Phosphorescence
C12N 5/00 (2006.01)
G01N 21/64 (2006.01)
CPC
C12N 2500/02
C12N 5/0062
C12N 5/0662
C12N 5/0663
C12N 5/0664
C12N 5/0665
Applicants
  • MEDIZINISCHE HOCHSCHULE HANNOVER [DE/DE]; Carl-Neuberg-Strasse 1 30625 Hannover, DE (AllExceptUS)
  • BIOMIMETICS TECHNOLOGIES INC. [CA/CA]; 191 Ellis Avenue Toronto, Ontario M6S2X4, CA (AllExceptUS)
  • STOLZ, Marvin [DE/DE]; DE (AllExceptUS)
  • SCHMÄLZLIN, Elmar [DE/DE]; DE
  • WEYAND, Birgit [DE/DE]; DE (UsOnly)
  • ISRAELOWITZ, Meir [CA/CA]; CA (UsOnly)
  • VOGT, Peter [DE/DE]; DE (UsOnly)
  • REIMERS, Kerstin [DE/DE]; DE (UsOnly)
  • VON SCHROEDER, Herbert, P. [CA/CA]; CA (UsOnly)
  • GILLE, Christoph [DE/CA]; CA (UsOnly)
Inventors
  • SCHMÄLZLIN, Elmar; DE
  • WEYAND, Birgit; DE
  • ISRAELOWITZ, Meir; CA
  • VOGT, Peter; DE
  • REIMERS, Kerstin; DE
  • VON SCHROEDER, Herbert, P.; CA
  • GILLE, Christoph; CA
Agents
  • TARUTTIS, Stefan; Aegidientorplatz 2b 30159 Hannover, DE
Priority Data
61/404,32004.10.2010US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) PROCESS FOR PRODUCING THREE-DIMENSIONAL TISSUE
(FR) PROCÉDÉ DE PRODUCTION D'UN TISSU TRIDIMENSIONNEL
Abstract
(EN)
The invention provides a process for producing a three-dimensional tissue by cultivating eucaryotic cells by introducing the cells into a matrix and cultivating the cells within the matrix in a cell culture medium within a cell culture vessel under controlled dissolved oxygen conditions of the cell culture medium. The matrix contains a support containing an optical oxygen sensor, which is an oxygen-sensitive dye, which upon irradiation with an excitation wavelength changes its emission characteristics in dependence on the dissolved oxygen concentration in the surrounding medium, including a dye phosphorescing upon irradiation of an excitation wavelength, which phosphorescence is quenched by dissolved oxygen.
(FR)
L'invention concerne un procédé de production d'un tissu tridimensionnel par mise en culture de cellules eucaryotes par introduction des cellules à l'intérieur d'une matrice et mise en culture des cellules au sein de la matrice dans un milieu de culture cellulaire, au sein d'un récipient de culture cellulaire, dans des conductions régulées d'oxygène dissous du milieu de culture cellulaire. La matrice contient un support contenant un capteur optique d'oxygène, qui est un colorant sensible à l'oxygène, qui, suite à l'irradiation par une longueur d'onde d'excitation, modifie ses caractéristiques d'émission en fonction de la concentration en oxygène dissous dans le milieu environnant, notamment un colorant phosphorescent sous irradiation à une longueur d'onde d'excitation, dont la phosphorescence est inactivée par l'oxygène dissous.
Also published as
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