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1. (WO2012045668) METHOD FOR CELL LYSIS IN A RT-PCR REACTION BUFFER
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2012/045668    International Application No.:    PCT/EP2011/067067
Publication Date: 12.04.2012 International Filing Date: 30.09.2011
IPC:
C12Q 1/68 (2006.01)
Applicants: ROCHE DIAGNOSTICS GMBH [DE/DE]; Sandhofer Straße 116 68305 Mannheim (DE) (DE only).
F. HOFFMANN-LA ROCHE AG [CH/CH]; Grenzacher Strasse 124 CH-4070 Basel (CH) (AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BE, BF, BG, BH, BJ, BR, BW, BY, BZ, CA, CF, CG, CH, CI, CL, CM, CN, CO, CR, CU, CY, CZ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, FR, GA, GB, GD, GE, GH, GM, GN, GQ, GR, GT, GW, HN, HR, HU, ID, IE, IL, IN, IS, IT, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LV, LY, MA, MC, MD, ME, MG, MK, ML, MN, MR, MT, MW, MX, MY, MZ, NA, NE, NG, NI, NL, NO, NZ, OM, PE, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SI, SK, SL, SM, SN, ST, SV, SY, SZ, TD, TG, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, UZ, VC, VN, ZA, ZM, ZW only).
HOFFMANN, Ingrid [DE/DE]; (DE) (For US Only).
WALCH, Heiko [DE/DE]; (DE) (For US Only)
Inventors: HOFFMANN, Ingrid; (DE).
WALCH, Heiko; (DE)
Agent: HILDEBRANDT, Martin; P.O.Box 11 52 Nonnenwald 2 82372 Penzberg (DE)
Priority Data:
10186417.1 04.10.2010 EP
10186416.3 04.10.2010 EP
Title (EN) METHOD FOR CELL LYSIS IN A RT-PCR REACTION BUFFER
(FR) MÉTHODE DE LYSE CELLULAIRE DANS UN TAMPON DE RÉACTION DE RT-PCR
Abstract: front page image
(EN)The present invention in general provides a method for amplification of a target RNA, comprising the steps of (i) transfering a liquid with a first volume comprising at least one or more living cells into a vessel (ii) adding to said vessel an RT-PCR reaction buffer with a second volume, whereas said second volume is at least 2x as large as said first volume (iii) lysing said at least one or more living cells within said vessel by means of incubation for at least 20 seconds at at least 90°C, and (iv) amplifying said target by means of a one-step RT-PCR without performance of an intermediate purification step.
(FR)La présente invention concerne en général un procédé d'amplification d'un ARN cible, comprenant les étapes de (i) transfert dans un récipient d'un liquide ayant un premier volume comprenant au moins une ou plusieurs cellules vivantes, (ii) ajout audit récipient d'un tampon de réaction de RT-PCR ayant un second volume, ledit second volume étant au moins 2x plus grand que ledit premier volume, (iii) lyse de ladite ou desdites cellules vivantes à l'intérieur dudit récipient au moyen d'une incubation pendant au moins 20 secondes à au moins 90°C et (iv) amplification de ladite cible au moyen d'une RT-PCR à étape unique sans mise en oeuvre d'une étape de purification intermédiaire.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)