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Machine translation
1. (WO2012044991) PURIFICATION OF NUCLEIC ACIDS
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2012/044991    International Application No.:    PCT/US2011/054322
Publication Date: 05.04.2012 International Filing Date: 30.09.2011
IPC:
B01L 3/02 (2006.01)
Applicants: PHYNEXUS, INC. [US/US]; 3670 Charter Park Dr., Suite A San Jose, CA 95136 (US) (For All Designated States Except US).
SUH, Chris [US/US]; (US).
HOANG, Lee [US/US]; (US).
GJERDE, Douglas, T. [US/US]; (US)
Inventors: SUH, Chris; (US).
HOANG, Lee; (US).
GJERDE, Douglas, T.; (US)
Agent: KALMAN, Sue; PhyNexus, Inc. 3670 Charter Park Dr., Suite A San Jose, CA 95136 (US)
Priority Data:
PCT/US2011/030232 29.03.2011 US
61/414,855 17.11.2010 US
61/388,460 30.09.2010 US
Title (EN) PURIFICATION OF NUCLEIC ACIDS
(FR) PURIFICATION D'ACIDES NUCLÉIQUES
Abstract: front page image
(EN)The present invention solves the problem of isolating nucleic acids from cells in the presence of the growth medium. The invention is particularly useful for isolating extrachromosomal replicons such as plasmids. Cells are lysed in the presence of the medium in which they were grown and nucleic acids are isolated using a pipette tip column. A liquid handling robot can be used to isolate nucleic acids from multiple samples simultaneously without the need for human intervention.
(FR)La présente invention concerne l'extraction d'acides nucléiques de cellules en présence du milieu de croissance. L'invention est particulièrement utile pour extraire des réplicons extrachromosomiques, tels que des plasmides. Les cellules sont soumises à une lyse en présence du milieu dans lequel elles ont été cultivées et les acides nucléiques sont isolés au moyen d'une colonne à embout de pipette. Un robot manipulateur de liquide peut être utilisé pour isoler simultanément les acides nucléiques de plusieurs échantillons, sans intervention humaine.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)