WIPO logo
Mobile | Deutsch | Español | Français | 日本語 | 한국어 | Português | Русский | 中文 | العربية |
PATENTSCOPE

Search International and National Patent Collections
World Intellectual Property Organization
Search
 
Browse
 
Translate
 
Options
 
News
 
Login
 
Help
 
Machine translation
1. (WO2012042053) METHOD OF SEQUENCING
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2012/042053    International Application No.:    PCT/EP2011/067201
Publication Date: 05.04.2012 International Filing Date: 30.09.2011
IPC:
C12Q 1/68 (2006.01)
Applicants: GeneSeque AS [NO/NO]; Richard Birkelands vei 2B N-7941 Trondheim (NO) (For All Designated States Except US).
KOTSBAK, Jarle [NO/NO]; (NO) (For US Only)
Inventors: KOTSBAK, Jarle; (NO)
Agent: JONES, Elizabeth; St. Bride's House 10 Salisbury Square London Greater London EC4Y 8JD (GB)
Priority Data:
1016484.6 30.09.2010 GB
Title (EN) METHOD OF SEQUENCING
(FR) MÉTHODE DE SÉQUENÇAGE
Abstract: front page image
(EN)The present invention provides a method of sequencing a polynucleotide using a stepwise ligation and cleavage method in which two or more cleavage enzymes which are specific to two or more test probes are used sequentially to identify which test probe bound and hence the sequence of the target polynucleotide. In a preferred aspect the probes and/or bases used in the reaction are labelled, e.g. with a bead.
(FR)L'invention concerne une méthode de séquençage d'un polynucléotide qui utilise un procédé de ligature et de clivage par étapes, dans lequel au moins deux enzymes de clivage, spécifiques d'au moins deux sondes de test, sont utilisées séquentiellement pour identifier quelle sonde de test a été liée, et par conséquent la séquence du polynucléotide cible. Dans un aspect préféré, les sondes et/ou les bases utilisées dans la réaction sont marquées, par. ex., au moyen d'une bille.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)