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1. (WO2012037937) METHOD FOR DETECTING, DIFFERENTIATING, AND QUANTIFYING T CELL POPULATIONS BY MEANS OF REVERSE TRANSCRIPTION QUANTITATIVE REAL-TIME PCR (RT-QPCR) TECHNOLOGY

Pub. No.:    WO/2012/037937    International Application No.:    PCT/DE2011/075225
Publication Date: Fri Mar 30 01:59:59 CEST 2012 International Filing Date: Sat Sep 17 01:59:59 CEST 2011
IPC: G01N 33/68
G01N 33/50
C12Q 1/68
Applicants: LOPHIUS BIOSCIENCES GMBH
DEML, Ludwig
NAUMANN, Kristina
SCHLOMBS, Kornelia
BARABAS, Sascha
Inventors: DEML, Ludwig
NAUMANN, Kristina
SCHLOMBS, Kornelia
BARABAS, Sascha
Title: METHOD FOR DETECTING, DIFFERENTIATING, AND QUANTIFYING T CELL POPULATIONS BY MEANS OF REVERSE TRANSCRIPTION QUANTITATIVE REAL-TIME PCR (RT-QPCR) TECHNOLOGY
Abstract:
The invention relates to a method for detecting, differentiating, and quantifying T cell populations, comprising the following steps: a) bringing a first aliquot of a bodily fluid of an individual in contact with at least one antigen, wherein the bodily fluid contains antigen-presenting cells (APCs) and T cells, b) incubating the first aliquot with the at least one antigen over a certain time period, c) detecting and differentiating the T cell populations by detecting at least one first marker of the APCs induced by the T cells of a certain T cell population in the first aliquot and in a second aliquot of the bodily fluid of the individual, which was not incubated with the at least one antigen, by means of reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR), and d) detecting and quantifying the T cell populations by determining the ratio of the detected markers of the APCs of the first aliquot to the second aliquot. The invention further relates to a kit for carrying out the method.