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120. (WO2012032521) NOVEL METHODS AND CULTURE MEDIA FOR CULTURING PLURIPOTENT STEM CELLS
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2012/032521 International Application No.: PCT/IL2011/000722
Publication Date: 15.03.2012 International Filing Date: 07.09.2011
IPC:
C12N 5/0735 (2010.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
073
Embryonic cells or tissues; Foetal cells or tissues
0735
Embryonic stem cells; Embryonic germ cells
Applicants:
AMIT, Michal [IL/IL]; IL (UsOnly)
ITSKOVITZ-ELDOR, Joseph [IL/IL]; IL (UsOnly)
TECHNION RESEARCH & DEVELOPMENT FOUNDATION LIMITED. [IL/IL]; Senate House Technion City 3200004 Haifa, IL (AllExceptUS)
Inventors:
AMIT, Michal; IL
ITSKOVITZ-ELDOR, Joseph; IL
Agent:
G.E. EHRLICH (1995) LTD.; 11 Menachem Begin Road 52681 Ramat Gan, IL
Priority Data:
61/380,38807.09.2010US
Title (EN) NOVEL METHODS AND CULTURE MEDIA FOR CULTURING PLURIPOTENT STEM CELLS
(FR) NOUVEAUX PROCÉDÉS ET MILIEUX DE CULTURE DESTINÉS À LA CULTURE DE CELLULES SOUCHES PLURIPOTENTES
Abstract:
(EN) Provided is an isolated population of human pluripotent stem cells comprising at least 50% human pluripotent stem cells characterized by an OCT4+/TRA1-60-/TRA1-81-/SSEA1+/SSEA4- expression signature, and novel methods of generating and maintaining same in a pluripotent, undifferentiated state a suspension culture devoid of cell clumps. Also provided are novel culture media for culturing pluripotent stem cells such as embryonic stem cells and induced pluripotent stem cells in a suspension culture or a two-dimensional culture system while maintaining the cells in a proliferative, pluripotent and undifferentiated state. The novel culture media comprise interleukin 11 (IL11) and Ciliay Neurotrophic Factor (CNTF); bFGF at a concentration of at least 50 ng/ml and an IL6RIL6 chimera; or an animal contaminant-free serum replacement and an IL6RIL6 chimera. Also provided are cell cultures comprising the pluripotent stem cells and the culture media and methods of using same for expanding and maintaining pluripotent stem cells in an undifferentiated state, or for generating lineage-specific cells from the pluripotent stem cells.
(FR) L'invention concerne une population isolée de cellules souches pluripotentes humaines comprenant au moins 50% de cellules souches pluripotentes humaines, caractérisées par une signature d'expression OCT4+/TRA1-60/TRA1-81-/SSEA1+/SSEA4-. Elle concerne de nouveaux procédés de production et de maintien desdites cellules souches, dans un état indifférencié pluripotent, dans une culture en suspension exempte d'amas de cellules. L'invention concerne également de nouveaux milieux de culture destinés à la culture de cellules souches pluripotentes, telles que des cellules souches embryonnaires et des cellules souches pluripotentes, induites dans un système de culture en suspension ou de culture bidimensionnelle avec maintien des cellules dans un état prolifératif, pluripotent et indifférencié. Ces nouveaux milieux de culture comprennent: l'interleukine 11 (IL11) et le facteur neurotrophique ciliaire (CNTF); bFGF à une concentration d'au moins 50 ng/ml et une chimère IL6RIL6; ou un sérum de remplacement exempt de contaminants d'origine animale et une chimère IL6RIL6. L'invention concerne en outre des cultures cellulaires comprenant les cellules souches pluripotentes et les milieux de culture de l'invention, et leurs procédés d'utilisation pour multiplier et maintenir des cellules souches pluripotentes dans un état indifférencié, ou pour produire des cellules spécifiques de lignées à partir des cellules souches pluripotentes.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)