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1. (WO2011151964) METHOD FOR DETECTING ANTIGENS

Pub. No.:    WO/2011/151964    International Application No.:    PCT/JP2011/001989
Publication Date: Dec 8, 2011 International Filing Date: Apr 1, 2011
IPC: G01N 27/416
G01N 27/327
G01N 33/543
Applicants: PANASONIC CORPORATION
パナソニック株式会社
NISHIO, Kazuaki
西尾 和晃
MATSUKAWA, Nozomu
松川 望
YOSHII, Shigeo
吉井 重雄
Inventors: NISHIO, Kazuaki
西尾 和晃
MATSUKAWA, Nozomu
松川 望
YOSHII, Shigeo
吉井 重雄
Title: METHOD FOR DETECTING ANTIGENS
Abstract:
Provided is a method for detecting antigens that does not require labeled antibodies. A carrier having antibodies and a multicopper oxidase (CueO) immobilized thereupon is brought into contact with a first buffer solution containing antigens, current is measured by a potentiostatic technique using the carrier and a second buffer solution, and when the measured current is 1.5 × (the blank value) or more, it is determined that antigens are present. The second buffer solution contains the CueO substrate, and has an ionic strength that is within a range of 0.3 mM to 1.0 mM.