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Pub. No.:    WO/2011/145211    International Application No.:    PCT/JP2010/058631
Publication Date: 24.11.2011 International Filing Date: 21.05.2010
C12N 15/09 (2006.01), C12N 1/15 (2006.01), C12N 1/19 (2006.01), C12N 1/21 (2006.01), C12N 5/10 (2006.01), C12N 9/02 (2006.01), C12N 15/00 (2006.01), C12P 19/56 (2006.01), C12P 21/02 (2006.01)
Applicants: Meiji Seika Pharma Co., Ltd. [JP/JP]; 4-16, Kyobashi 2-chome, Chuo-ku, Tokyo 1048002 (JP) (For All Designated States Except US).
MAZUKA Fusuke [JP/JP]; (JP) (For US Only).
FUKUSHIMA Takayoshi [JP/JP]; (JP) (For US Only).
SUMIDA Naomi [JP/JP]; (JP) (For US Only).
YANAI Koji [JP/JP]; (JP) (For US Only)
Inventors: MAZUKA Fusuke; (JP).
FUKUSHIMA Takayoshi; (JP).
SUMIDA Naomi; (JP).
YANAI Koji; (JP)
Agent: MORITA Kenichi; 5th Fl., Itabashi-chuo Bldg., 67-8, Itabashi 2-chome, Itabashi-ku, Tokyo 1730004 (JP)
Priority Data:
(JA) ケトレダクターゼ変異体
Abstract: front page image
(EN)Disclosed are: a ketoreductase mutant which can be used for the efficient production of a daunorubicin derivative; DNA which encodes the mutant; a transformant which has the DNA introduced therein and therefore can produce a daunorubicin derivative; and a process for producing a daunorubicin derivative using the transformant. The ketoreductase mutant comprises an amino acid sequence produced by substituting at least one amino acid residue selected from the group consisting of amino acid residues located at positions corresponding to 42nd, 149th, 153rd, 270th and 306th amino acid residues in the amino acid sequence for a ketoreductase (EvaE) from a chloroeremomycin-producing bacterium (Amycolatopsis orientalis) by another amino acid residue.
(FR)La présente invention concerne : un mutant de cétone réductase pouvant être mis en œuvre pour une production efficace de dérivé de daunorubicine; un ADN codant pour ledit mutant; un transformant qui par transfert dans celui-ci dudit ADN, produit le dérivé de daunorubicine; et le procédé de fabrication d'un dérivé de daunorubicine utilisant ledit transformant. Ledit mutant de cétone réductase présente un ou plusieurs résidus d'acide aminé choisis parmi un groupe constitué d'acides aminés de positions correspondant aux acides aminés 42, 149, 153, 270 et 306 dans une séquence d'acides aminés de cétone réductase (EvaE) de bactérie produisant un chloroérémomycine (Amycolatopsis orientalis), lesquels résidus sont substitués par d'autres résidus d'acide aminé.
(JA) ダウノルビシン誘導体の効率的な生産に利用可能なケトレダクターゼ変異体、該変異体をコードするDNA、該DNAが導入されてダウノルビシン誘導体を生産する形質転換体、及び該形質転換体を用いたダウノルビシン誘導体の製造方法を開示する。 前記ケトレダクターゼ変異体は、クロロレモマイシン生産菌(Amycolatopsis orientalis)のケトレダクターゼ(EvaE)のアミノ酸配列中の42、149、153、270および306番目のアミノ酸に相当する位置のアミノ酸からなる群から選択される1個または2個以上のアミノ酸残基が、他のアミノ酸残基により置換されてなる。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)