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1. (WO2011143802) METHOD FOR IN VITRO INDUCING MEGAKARYOCYTE PROGENITOR CELLS AND MEGAKARYOCYTES
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2011/143802    International Application No.:    PCT/CN2010/002181
Publication Date: 24.11.2011 International Filing Date: 28.12.2010
IPC:
C12N 5/0786 (2010.01), C12N 5/078 (2010.01)
Applicants: INSTITUE OF TRANSFUSION MEDICINE, ACADEMY OF MILITARY MEDICAL SCIENCES, PEOPLE'S LIBRATION ARMY OF CHINA [CN/CN]; No.27, Tai Ping Road, Haidian District Beijing 100850 (CN) (For All Designated States Except US).
PEI, Xuetao [CN/CN]; (CN) (For US Only).
CHEN, Lin [CN/CN]; (CN) (For US Only).
SHI, Wei [CN/CN]; (CN) (For US Only).
LIU, Daqing [CN/CN]; (CN) (For US Only).
YUE, Wen [CN/CN]; (CN) (For US Only).
LV, Yang [CN/CN]; (CN) (For US Only).
LI, Yanhua [CN/CN]; (CN) (For US Only).
XIE, Xiaoyan [CN/CN]; (CN) (For US Only).
ZHU, Honghu [CN/CN]; (CN) (For US Only)
Inventors: PEI, Xuetao; (CN).
CHEN, Lin; (CN).
SHI, Wei; (CN).
LIU, Daqing; (CN).
YUE, Wen; (CN).
LV, Yang; (CN).
LI, Yanhua; (CN).
XIE, Xiaoyan; (CN).
ZHU, Honghu; (CN)
Agent: SHANGCHENG & PARTNERS; Suite 1801 18th Floor, Ever Bright World Center No. 28 West Ping An Li Avenue Xicheng District Beijing 100034 (CN)
Priority Data:
201010178856.X 17.05.2010 CN
Title (EN) METHOD FOR IN VITRO INDUCING MEGAKARYOCYTE PROGENITOR CELLS AND MEGAKARYOCYTES
(FR) PROCÉDÉ POUR INDUIRE IN VITRO DES CELLULES PROGÉNITRICES DE MÉGAKARYOCYTES ET DES MÉGAKARYOCYTES
(ZH) 一种体外诱导巨核祖细胞和巨核细胞的方法
Abstract: front page image
(EN)A method for in vitro inducing megakaryocyte progenitor cells and megakaryocytes and special culture medium thereof are provided. The special culture medium is the StemSpan® serum-free medium comprising 50 ng/ml of SCF, 50 ng/ml of TPO, 20 ng/ml of IL-3, and 50 ng/ml of IL-6. The method comprises following steps: 1) separating single nuclear cells from umbilical cord bloods by the normal Ficoll density gradient centrifugation method, wherein red cells in umbilical cord bloods are settled by hydroxyethyl starch having the concentration of 6%; 2) culturing the single nuclear cells in the special culture medium for 4-14 days in vitro under the condition of 37℃, 5% CO2 to obtain the mixture of megakaryocyte progenitor cells and megakaryocytes. The method is simple in operation, low in cost, and high in differentiation efficiency.
(FR)L'invention concerne un procédé pour induire in vitro des cellules progénitrices de mégakaryocytes, des mégakaryocytes et un milieu de culture spécial qui est le milieu StemSpan®sans sérum comprenant 50 ng/ml de SCF, 50 ng/ml de TPO, 20 ng/ml de IL-3 et 50 ng/ml de IL-6. Le procédé comprend les étapes consistant : 1) à séparer des cellules nucléaires seules provenant de sangs de cordon ombilical au moyen d'un procédé de centrifugation en gradient de densité Ficoll normal, les globules rouges des sangs de cordon ombilical étant déposées au moyen d'amidon hydroxyéthylé dont la concentration est de 6%; 2) à cultiver in vitro les cellules nucléaires seules dans le milieu de culture spécial pendant 4-14 jours dans conditions de 37°C et de 5% CO2 pour obtenir le mélange de cellules progénitrices de mégakaryocytes et de mégakaryocytes. Ce procédé présente un fonctionnement simple, un faible coût et une efficacité élevée.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)