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1. (WO2011142492) DNA POLYMERASE CAPABLE OF LONG PCR, AND GENES THEREOF
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2011/142492    International Application No.:    PCT/KR2010/003074
Publication Date: 17.11.2011 International Filing Date: 14.05.2010
IPC:
C12N 9/12 (2006.01), C12N 15/54 (2006.01), C12N 15/63 (2006.01)
Applicants: KOREA OCEAN RESEARCH & DEVELOPMENT INSTITUTE [KR/KR]; #1270 Sa 2-dong, Sangnok-gu Ansan Si, Gyeonggi-Do 426-744 (KR) (For All Designated States Except US).
LEE, Jung Hyun [KR/KR]; (KR) (For US Only).
KANG, Sung Gyun [KR/KR]; (KR) (For US Only).
KIM, Sang Jin [KR/KR]; (KR) (For US Only).
KWON, Kae Kyoung [KR/KR]; (KR) (For US Only).
LEE, Hyun Sook [KR/KR]; (KR) (For US Only).
KIM, Yun Jae [KR/KR]; (KR) (For US Only).
BAE, Seung Seop [KR/KR]; (KR) (For US Only).
LIM, Jae Kyu [KR/KR]; (KR) (For US Only).
JEON, Jung Ho [KR/KR]; (KR) (For US Only).
CHO, Yona [KR/KR]; (KR) (For US Only).
HWANG, Young Ok [KR/KR]; (KR) (For US Only).
CHA, Sun Shin [KR/KR]; (KR) (For US Only)
Inventors: LEE, Jung Hyun; (KR).
KANG, Sung Gyun; (KR).
KIM, Sang Jin; (KR).
KWON, Kae Kyoung; (KR).
LEE, Hyun Sook; (KR).
KIM, Yun Jae; (KR).
BAE, Seung Seop; (KR).
LIM, Jae Kyu; (KR).
JEON, Jung Ho; (KR).
CHO, Yona; (KR).
HWANG, Young Ok; (KR).
CHA, Sun Shin; (KR)
Agent: YOON, Yuh Gang; 307Ho, Theosuperium 1Danji, 856-5 Nakseongdae-dong, Gwanak-gu Seoul 151-848 (KR)
Priority Data:
Title (EN) DNA POLYMERASE CAPABLE OF LONG PCR, AND GENES THEREOF
(FR) ADN POLYMÉRASE PERMETTANT UNE PCR LONGUE, ET GÈNES CORRESPONDANTS
(KO) 롱 PCR이 가능한 DNA 중합효소 및 이의 유전자들
Abstract: front page image
(EN)The present invention relates to a mutant DNA polymerase capable of long PCR, and to the genes thereof, and more specifically, to a DNA polymerase that is useful in long PCR polymerization obtained by mutating a wild-type TNA1_pol DNA polymerase derived from the Thermococcus sp. strain, to a gene encoding the mutant DNA polymerase, and to a method for preparing a polymerase using same. The mutant DNA polymerase of the present invention exhibits excellent performance in the PCR of long-chain DNA, compared to conventional wild-types or mutant-types, and thus may be widely applied in various fields that require the amplification of long chains, such as human gene vector analysis, etc.
(FR)L'invention concerne une ADN polymérase mutante permettant de mettre en œuvre une PCR longue et les gènes correspondants, et se réfère plus spécifiquement à une ADN polymérase qui est utile dans une polymérisation PCR longue, obtenue par la mutation d'une ADN polymérase TNA1_pol de type sauvage, dérivée de la souche Thermococcus sp., à un gène codant pour l'ADN polymérase mutante et à un procédé de préparation d'une polymérase utilisant celui-ci. L'ADN polymérase mutante invention présente une excellente efficacité dans la PCR d'ADN à chaînes longues par rapport à des types sauvages ou à des types mutants classiques, et peut par conséquent être largement appliquée dans divers domaines nécessitant l'amplification de chaînes longues, telle que l'analyse de vecteurs géniques humains, etc.
(KO)본 발명은 롱(long) PCR이 가능한 돌연변이 DNA 중합효소 및 이의 유전자들에 관한 것으로서, 보다 상세하게는 써모코커스 속 (Thermococcus sp.) 균주로부터 유래하고 야생형 TNA1_pol DNA 중합효소를 돌연변이화 하여 긴(long) PCR 중합반응에 유용한 DNA 중합효소, 상기 돌연변이 DNA 중합효소를 암호화하는 유전자 및 이를 이용한 중합효소 제조방법에 관한 것이다. 본 발명의 돌연변이 DNA 중합효소는 기존의 야생형이나 돌연변이형에 비해 긴(long) 사슬의 DNA에 대한 PCR에 있어서 월등한 성능을 나타냄으로써 인간의 유전체 분석 등 긴 사슬의 증폭이 필요한 다양한 분야에 널리 응용될 수 있다.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: Korean (KO)
Filing Language: Korean (KO)