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1. WO2011137533 - METHOD OF PROCESSING DRIED SAMPLES USING DIGITAL MICROFLUIDIC DEVICE

Publication Number WO/2011/137533
Publication Date 10.11.2011
International Application No. PCT/CA2011/050205
International Filing Date 15.04.2011
IPC
G01N 1/28 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
28Preparing specimens for investigation
H01J 49/04 2006.1
HELECTRICITY
01BASIC ELECTRIC ELEMENTS
JELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
49Particle spectrometers or separator tubes
02Details
04Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
G01N 27/00 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
27Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
CPC
B01L 2200/0631
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2200Solutions for specific problems relating to chemical or physical laboratory apparatus
06Fluid handling related problems
0631Purification arrangements, e.g. solid phase extraction [SPE]
B01L 2400/0427
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2400Moving or stopping fluids
04Moving fluids with specific forces or mechanical means
0403specific forces
0415electrical forces, e.g. electrokinetic
0427Electrowetting
B01L 3/502753
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
3Containers or dishes for laboratory use, e.g. laboratory glassware
50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
502with fluid transport, e.g. in multi-compartment structures
5027by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
502753characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
B01L 3/502784
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
3Containers or dishes for laboratory use, e.g. laboratory glassware
50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
502with fluid transport, e.g. in multi-compartment structures
5027by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
502769characterised by multiphase flow arrangements
502784specially adapted for droplet or plug flow, e.g. digital microfluidics
B01L 3/502792
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
3Containers or dishes for laboratory use, e.g. laboratory glassware
50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
502with fluid transport, e.g. in multi-compartment structures
5027by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
502769characterised by multiphase flow arrangements
502784specially adapted for droplet or plug flow, e.g. digital microfluidics
502792for moving individual droplets on a plate, e.g. by locally altering surface tension
G01N 1/28
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
28Preparing specimens for investigation ; including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
Applicants
  • THE GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTO [CA]/[CA] (AllExceptUS)
  • JEBRAIL, Mais, J. [CA]/[CA] (UsOnly)
  • YANG, Hao [CA]/[CA] (UsOnly)
  • WHEELER, Aaron, R. [US]/[CA] (UsOnly)
Inventors
  • JEBRAIL, Mais, J.
  • YANG, Hao
  • WHEELER, Aaron, R.
Agents
  • HILL & SCHUMACHER
Priority Data
61/331,67905.05.2010US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) METHOD OF PROCESSING DRIED SAMPLES USING DIGITAL MICROFLUIDIC DEVICE
(FR) PROCÉDÉ DE TRAITEMENT D'ÉCHANTILLONS SÉCHÉS UTILISANT UN DISPOSITIF MICROFLUIDIQUE NUMÉRIQUE
Abstract
(EN) Methods are provided for the preparation of a sample using a digital microfluidic platform and the optional subsequent mass analysis of an extracted analyte. A sample is dried, optionally on a solid phase support, and contacted with digital microfluidic array. An analyte present within the dried sample is extracted into an extraction solvent by electrically addressing the digital microfluidic array to transport a droplet of extraction solvent to the dried sample spot. The extracted sample may be dried and subsequently processed on the digital microfluidic array for derivatization. The digital microfluidic device may further include an integrated microfluidic channel having an output aperture, and the method may further include contacting a droplet containing extracted analyte with the microfluidic channel and applying a suitable electric field for generating nano- electrospray, thereby enabling the device to be directly interfaced with a mass analysis device.
(FR) L'invention concerne des procédés de préparation d'un échantillon utilisant une plateforme microfluidique numérique et l'analyse de masse ultérieure optionnelle d'un analyte extrait. Un échantillon est séché, éventuellement sur un support en phase solide, et mis en contact avec un réseau microfluidique numérique. Un analyte présent dans l'échantillon séché est extrait dans un solvant d'extraction par adressage électrique du réseau microfluidique numérique pour transporter une gouttelette du solvant d'extraction vers la pointe d'échantillon séché. L'échantillon extrait peut être séché et par la suite traité sur le réseau microfluidique numérique pour dérivation. Le dispositif microfluidique numérique peut en outre comporter un canal microfluidique solidaire présentant une ouverture de sortie, et le procédé peut également consister à mettre en contact une gouttelette contenant un analyte extrait avec le canal microfluidique et à appliquer un champ électrique approprié pour générer une nano-vaporisation, ce qui permet de mettre directement en interface le dispositif avec le dispositif d'analyse de masse.
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