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1. WO2011108687 - ESCHERICHIA COLI MEMBRANE PROTEIN INSERTION FACTOR

Publication Number WO/2011/108687
Publication Date 09.09.2011
International Application No. PCT/JP2011/055026
International Filing Date 04.03.2011
IPC
C12P 19/26 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
26Preparation of nitrogen-containing carbohydrates
C07H 13/06 2006.1
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
13Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids
02by carboxylic acids
04having the esterifying carboxyl radicals attached to acyclic carbon atoms
06Fatty acids
C08B 37/00 2006.1
CCHEMISTRY; METALLURGY
08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
BPOLYSACCHARIDES; DERIVATIVES THEREOF
37Preparation of polysaccharides not provided for in groups C08B1/-C08B35/110; Derivatives thereof
C12N 15/09 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
C12P 21/02 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21Preparation of peptides or proteins
02having a known sequence of two or more amino acids, e.g. glutathione
CPC
C07H 11/04
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
11Compounds containing saccharide radicals esterified by inorganic acids; Metal salts thereof
04Phosphates; Phosphites; Polyphosphates
C08B 37/006
CCHEMISTRY; METALLURGY
08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
BPOLYSACCHARIDES; DERIVATIVES THEREOF
37Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
006Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
C12P 21/02
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21Preparation of peptides or proteins
02having a known sequence of two or more amino acids, e.g. glutathione
Applicants
  • 公益財団法人サントリー生命科学財団 SUNTORY FOUNDATION FOR LIFE SCIENCES [JP]/[JP] (AllExceptUS)
  • 楠本 正一 KUSUMOTO, Shoichi [JP]/[JP] (UsOnly)
  • 島本 啓子 SHIMAMOTO, Keiko [JP]/[JP] (UsOnly)
  • 前田 将秀 MAEDA, Masahide [JP]/[JP] (UsOnly)
  • 西山 賢一 NISHIYAMA, Kenichi [JP]/[JP] (UsOnly)
  • 徳田 元 TOKUDA, Hajime [JP]/[JP] (UsOnly)
  • 上田 卓也 UEDA, Takuya [JP]/[JP] (UsOnly)
  • 金森 崇 KANAMORI, Takashi [JP]/[JP] (UsOnly)
Inventors
  • 楠本 正一 KUSUMOTO, Shoichi
  • 島本 啓子 SHIMAMOTO, Keiko
  • 前田 将秀 MAEDA, Masahide
  • 西山 賢一 NISHIYAMA, Kenichi
  • 徳田 元 TOKUDA, Hajime
  • 上田 卓也 UEDA, Takuya
  • 金森 崇 KANAMORI, Takashi
Agents
  • 草間 攻 KUSAMA, Osamu
Priority Data
2010-04852005.03.2010JP
2010-05175009.03.2010JP
Publication Language Japanese (ja)
Filing Language Japanese (JA)
Designated States
Title
(EN) ESCHERICHIA COLI MEMBRANE PROTEIN INSERTION FACTOR
(FR) FACTEUR D'INSERTION DE PROTÉINES MEMBRANAIRES D'ESCHERICHIA COLI
(JA) 大腸菌膜タンパク質挿入因子
Abstract
(EN) Disclosed are: a novel Escherichia coli membrane protein insertion factor which is involved in the intake of a protein; and a method for utilizing the Escherichia coli membrane protein insertion factor particularly in the synthesis of a membrane protein in a cell-free system and the insertion of a protein into a membrane which occurs in conjunction with the aforementioned synthesis. Specifically disclosed is a membrane protein insertion factor in a highly purified form, which is characterized by having a structure represented by chemical formula (Ia) or (II). (In the formulae, R1 represents a saturated /unsaturated fatty acid residue that has a carbon chain containing 16 to 20 carbon atoms; R2 represents a hydrogen atom or an acetyl group; n represents an integer of 20 or less; GlcNAc represents N-acetylglucosamine; ManNAcA represents N-acetylmannosamine uronic acid; and Fuc4NAc represents 4-N-acetylaminofucose.)
(FR) La présente invention concerne : un nouveau facteur d'insertion de protéines membranaires d'Escherichia coli qui est impliqué dans l'absorption d'une protéine; et un procédé destiné à utiliser le facteur d'insertion de protéines membranaires d'Escherichia coli en particulier dans la synthèse d'une protéine membranaire dans un système exempt de cellules et l'insertion d'une protéine à l'intérieur d'une membrane qui a lieu en association avec la synthèse mentionnée précédemment. L'invention concerne spécifiquement un facteur d'insertion de protéines membranaires sous une forme hautement purifiée, qui est caractérisé en ce qu'il a une structure représentée par les formules chimiques (Ia) ou (II). (Dans les formules, R1 représente un résidu acide gras saturé/insaturé ayant une chaîne carbonée contenant de 16 à 20 atomes de carbone; R2 représente un atome d'hydrogène ou un groupe acétyle; n représente un entier inférieur ou égal à 20; GlcNAc représente une N-acétylglucosamine; ManNAcA représente un acide N-acétylmannosamine-uronique; et Fuc4NAc représente le 4-N-acétylaminofucose.)
(JA)  タンパク質取り込みに関わる新規な大腸菌膜タンパク質挿入因子を提供すること、並びに、特に無細胞系中での膜タンパク質合成およびそれの共役するタンパク質の膜挿入におけるその使用方法を提供するものであり、高度に精製された形態の膜タンパク質挿入因子であって、下記化学式(Ia)または(II)に示す構造を有することを特徴とする膜タンパク質挿入因子である。(但し、式中、Rは炭素鎖16~20の飽和/不飽和脂肪酸残基を表し、Rは水素原子またはアセチル基を表し、nは20以下の整数である。 また、GlcNAcはN-アセチルグルコサミンを、ManNAcAはN-アセチルマンノサミンウロン酸を、Fuc4NAcは4-N-アセチルアミノフコースを意味する)
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