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1. (WO2011096223) INDUCED HEPATIC STEM CELL AND PROCESS FOR PRODUCTION THEREOF, AND APPLICATIONS OF THE CELL
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2011/096223 International Application No.: PCT/JP2011/000621
Publication Date: 11.08.2011 International Filing Date: 03.02.2011
IPC:
C12N 5/10 (2006.01) ,A01K 67/027 (2006.01) ,A61K 35/12 (2006.01) ,C12N 5/071 (2010.01) ,C12P 21/02 (2006.01) ,C12Q 1/02 (2006.01) ,C12N 15/12 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
10
Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
A HUMAN NECESSITIES
01
AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
K
ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
67
Rearing or breeding animals, not otherwise provided for; New breeds of animals
027
New breeds of vertebrates
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
K
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35
Medicinal preparations containing material or reaction products thereof with undetermined constitution
12
Materials from mammals or birds
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21
Preparation of peptides or proteins
02
having a known sequence of two or more amino acids, e.g. glutathione
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
02
involving viable micro-organisms
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
12
Genes encoding animal proteins
Applicants:
独立行政法人国立がん研究センター NATIONAL CANCER CENTER [JP/JP]; 東京都中央区築地5-1-1 5-1-1,Tsukiji, Chuo-ku, Tokyo 1040045, JP (AllExceptUS)
石川哲也 ISHIKAWA, Tetsuya [JP/JP]; JP (UsOnly)
萩原啓太郎 HAGIWARA, Keitaro [JP/JP]; JP (UsOnly)
落谷孝広 OCHIYA, Takahiro [JP/JP]; JP (UsOnly)
Inventors:
石川哲也 ISHIKAWA, Tetsuya; JP
萩原啓太郎 HAGIWARA, Keitaro; JP
落谷孝広 OCHIYA, Takahiro; JP
Agent:
滝田清暉 TAKITA, Seiki; 東京都新宿区歌舞伎町2-41-12 岡埜ビル7階IP国際技術特許事務所 IP.KOKUSAI PAT&ENG. 7th Floor, Okano-bldg., 41-12, Kabukicho 2-chome, Shinjuku-ku, Tokyo 1600021, JP
Priority Data:
2010-02260003.02.2010JP
Title (EN) INDUCED HEPATIC STEM CELL AND PROCESS FOR PRODUCTION THEREOF, AND APPLICATIONS OF THE CELL
(FR) CELLULE SOUCHE HÉPATIQUE INDUITE ET PROCÉDÉ POUR LA PRODUCTION DE CELLE-CI, ET APPLICATIONS DE LA CELLULE
(JA) 誘導肝幹細胞及びその製造方法、並びに、該細胞の応用
Abstract:
(EN) Disclosed are: an induced hepatic stem cell useful for safety tests, toxicity tests, metabolism tests, drug interaction tests, anti-viral activity tests, screening tests for medicinal agents such as hyperlipemia therapeutic agents, hypertension therapeutic agents, pharmaceutical low-molecular-weight compound agents and pharmaceutical antibody agents, the screening for potential drug targets, the production of animal models, the production of hepatocyte-produced proteins, and regenerative medicine; a process for producing the cell; and applications of the cell. The induced hepatic stem cell is characterized by fulfilling at least the following requirements (1) to (3): (1) at least 15 genes selected from specific genes that are marker genes for embryonic stem cells are expressed in the cell; (2) the cell has properties of hepatocytes; and (3) the cell can be proliferatively cultured or sub-cultured for at least three days.
(FR) La présente invention concerne : une cellule souche hépatique induite utile pour des essais de sécurité, des essais de toxicité, des essais de métabolisme, des essais d'interaction médicamenteuse, des essais d'activité antivirale, des essais de criblage pour des agents médicinaux tels que des agents thérapeutiques contre l'hyperlipidémie, des agents thérapeutiques contre l'hypertension, des agents pharmaceutiques de composé de faible poids moléculaire et des agents pharmaceutiques d'anticorps, le criblage de cibles médicamenteuses potentielles, la production de modèles animaux, la production de protéines produites par des hépatocytes, et la médecine régénérative ; un procédé pour produire la cellule ; et des applications de la cellule. La cellule souche hépatique induite est caractérisée en ce qu'elle satisfait au moins aux exigences suivantes (1) à (3) : (1) au moins 15 gènes choisis parmi des gènes spécifiques qui sont des gènes marqueurs pour des cellules souches embryonnaires sont exprimés dans la cellule ; (2) la cellule a des propriétés d'hépatocytes ; et (3) la cellule peut être cultivée de façon proliférative ou sous-cultivée pendant au moins trois jours.
(JA)  本発明は、安全性試験、毒性試験、代謝試験、薬物相互作用試験、抗ウイルス活性試験、高脂血症薬、高血圧治療薬、低分子化合物医薬、抗体医薬などの医薬品のスクリーニング試験、創薬標的スクリーニング、動物モデル作製、肝細胞産生タンパク質の生産、及び再生医療に有用な、下記の誘導肝幹細胞、その製造方法及び該細胞の応用である。 少なくとも下記(1)~(3)の要件を具備することを特徴とする誘導肝幹細胞; (1)胚性幹細胞のマーカー遺伝子である特定の遺伝子群の中から選択された少なくとも15種の遺伝子を発現している (2)肝細胞としての性質を有する (3)3日以上増殖培養又は継代培養可能である。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)
Also published as:
EP2532741US20130023045JPWO2011096223CN102858958CA2794473KR1020130012114
JP2016010406IN7576/DELNP/2012ES2667058