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1. WO2011074564 - METHOD OF PRODUCING ADENOVIRUS VECTORS

Publication Number WO/2011/074564
Publication Date 23.06.2011
International Application No. PCT/JP2010/072450
International Filing Date 14.12.2010
IPC
C12N 15/00 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
A61K 35/76 2006.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35Medicinal preparations containing materials or reaction products thereof with undetermined constitution
66Microorganisms or materials therefrom
76Viruses; Subviral particles; Bacteriophages
A61K 48/00 2006.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
48Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
A61P 43/00 2006.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
43Drugs for specific purposes, not provided for in groups A61P1/-A61P41/108
C12N 15/09 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
CPC
A61K 35/00
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35Medicinal preparations containing materials or reaction products thereof with undetermined constitution
A61P 43/00
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
43Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
C12N 15/86
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
85for animal cells
86Viral vectors
C12N 2710/10343
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2710dsDNA Viruses
00011dsDNA Viruses
10011Adenoviridae
10311Mastadenovirus, e.g. human or simian adenoviruses
10341Use of virus, viral particle or viral elements as a vector
10343viral genome or elements thereof as genetic vector
C12N 2710/10351
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2710dsDNA Viruses
00011dsDNA Viruses
10011Adenoviridae
10311Mastadenovirus, e.g. human or simian adenoviruses
10351Methods of production or purification of viral material
Applicants
  • タカラバイオ株式会社 TAKARA BIO INC. [JP]/[JP] (AllExceptUS)
  • 蝶野 英人 CHONO, Hideto [JP]/[JP] (UsOnly)
  • 江頭 知恵 EGASHIRA, Tomoe [JP]/[JP] (UsOnly)
  • 吉岡 広文 YOSHIOKA, Hirofumi [JP]/[JP] (UsOnly)
  • 高蔵 晃 TAKAKURA, Hikaru [JP]/[JP] (UsOnly)
  • 峰野 純一 MINENO, Junichi [JP]/[JP] (UsOnly)
Inventors
  • 蝶野 英人 CHONO, Hideto
  • 江頭 知恵 EGASHIRA, Tomoe
  • 吉岡 広文 YOSHIOKA, Hirofumi
  • 高蔵 晃 TAKAKURA, Hikaru
  • 峰野 純一 MINENO, Junichi
Agents
  • 田中 光雄 TANAKA, Mitsuo
Priority Data
2009-28447515.12.2009JP
Publication Language Japanese (JA)
Filing Language Japanese (JA)
Designated States
Title
(EN) METHOD OF PRODUCING ADENOVIRUS VECTORS
(FR) PROCÉDÉ DE FABRICATION D'UN VECTEUR D'ADÉNOVIRUS
(JA) アデノウイルスベクターの製造方法
Abstract
(EN)
Disclosed is a method of producing adenovirus vectors which is characterized by involving a step (1) for culturing adenovirus vector-producing cells and producing adenovirus vectors, and a step (2) for treating with protease the adenovirus vectors obtained in step (1). By means of said method, adenovirus vectors with increased infection efficiency can be provided that are suitable for introducing foreign genes into cells.
(FR)
L'invention concerne un procédé de fabrication d'un vecteur d'adénovirus, caractérisé en ce qu'il comporte (1) une étape dans laquelle des cellules productrices du vecteur d'adénovirus sont mises en culture et le vecteur d'adénovirus est produit, et (2) une étape dans laquelle le vecteur d'adénovirus obtenu dans l'étape (1) est traité avec une protéase. Grâce à ce procédé de fabrication, on obtient un vecteur d'adénovirus adapté à l'introduction de gènes exogènes dans des cellules et pour lequel l'efficacité de transmission est augmentée.
(JA)
 本発明は、(1)アデノウイルスベクター産生細胞を培養し、アデノウイルスベクターを産生させる工程、及び(2)工程(1)で得られたアデノウイルスベクターをプロテアーゼで処理する工程、を包含することを特徴とするアデノウイルスベクターの製造方法に関する。該製造方法により、細胞に外来遺伝子を導入するのに適した、感染効率が向上したアデノウイルスベクターが提供される。
Also published as
Latest bibliographic data on file with the International Bureau