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1. WO2011035423 - METHOD OF PREPARING PLANT-DERIVED PROTEINS

Publication Number WO/2011/035423
Publication Date 31.03.2011
International Application No. PCT/CA2010/001489
International Filing Date 21.09.2010
IPC
C07K 1/14 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
1General processes for the preparation of peptides
14Extraction; Separation; Purification
C07K 1/34 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
1General processes for the preparation of peptides
14Extraction; Separation; Purification
34by filtration, ultrafiltration or reverse osmosis
C07K 14/415 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
415from plants
C12N 5/04 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
04Plant cells or tissues
C12N 15/00 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
C12N 15/82 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells
CPC
A61K 2039/517
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
39Medicinal preparations containing antigens or antibodies
51comprising whole cells, viruses or DNA/RNA
517Plant cells
A61K 2039/5258
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
39Medicinal preparations containing antigens or antibodies
51comprising whole cells, viruses or DNA/RNA
525Virus
5258Virus-like particles
A61K 39/12
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
39Medicinal preparations containing antigens or antibodies
12Viral antigens
A61K 39/145
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
39Medicinal preparations containing antigens or antibodies
12Viral antigens
145Orthomyxoviridae, e.g. influenza virus
A61P 31/00
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
31Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
A61P 31/16
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
31Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
12Antivirals
14for RNA viruses
16for influenza or rhinoviruses
Applicants
  • MEDICAGO INC. [CA]/[CA] (AllExceptUS)
  • VEZINA, Louis-Philippe [CA]/[CA] (UsOnly)
  • COUTURE, Manon [CA]/[CA] (UsOnly)
  • PAQUET, Dany [CA]/[CA] (UsOnly)
  • DARGIS, Michele [CA]/[CA] (UsOnly)
  • D'AOUST, Marc-Andre [CA]/[CA] (UsOnly)
Inventors
  • VEZINA, Louis-Philippe
  • COUTURE, Manon
  • PAQUET, Dany
  • DARGIS, Michele
  • D'AOUST, Marc-Andre
Agents
  • SECHLEY, Konrad, A.
Priority Data
61/244,78622.09.2009US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHOD OF PREPARING PLANT-DERIVED PROTEINS
(FR) PROCÉDÉ DE PRÉPARATION DE PROTÉINES DÉRIVÉES DE PLANTES
Abstract
(EN)
Methods of preparing plant-derived proteins or suprastructure proteins, are provided. The method may comprise obtaining a plant, or plant matter comprising apoplast-localized proteins, or suprastructure proteins, producing a protoplast/spheroplast fraction and apoplast fraction from the plant or plant matter, and recovering the apoplast fraction. The apoplast fraction comprises plant-derived proteins or suprastructure proteins,. Alternatively, the proteins, or suprastructure proteins, may be obtained from plant or plant matter comprising plant-derived proteins or suprastructure proteins, by digesting the plant matter using a cell wall degrading enzyme composition to produced a digested fraction. The digested fraction is filtered to produced a filtered fraction, and the plant-derived proteins or suprastructure proteins, are recovered from the filtered fraction.
(FR)
L'invention concerne des procédés de préparation de protéines ou protéines à suprastructure dérivées de plantes. Le procédé peut comprendre l'obtention d'une plante ou d'une matière végétale comprenant des protéines ou protéines à suprastructure localisées dans l'apoplaste, la production d'une fraction protoplaste/sphéroplaste et d'une fraction apoplaste à partir de la plante ou de la matière végétale, et la récupération de la fraction apoplaste. La fraction apoplaste comprend les protéines ou protéines à suprastructure dérivées de plantes. En variante, les protéines ou protéines à suprastructure peuvent être obtenues à partir d'une plante ou d'une matière végétale comprenant des protéines ou protéines à suprastructure dérivées de plantes par digestion de la matière végétale en utilisant une composition enzymatique de dégradation des parois cellulaires pour produire une fraction digérée. La fraction digérée est filtrée pour produire une fraction filtrée, et les protéines ou protéines à suprastructure dérivées de plantes sont récupérées à partir de la fraction filtrée.
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