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1. WO2011031025 - METHOD FOR DETECTING AND QUANTIFYING A TARGET SUBSTANCE USING A BIOCHIP

Publication Number WO/2011/031025
Publication Date 17.03.2011
International Application No. PCT/KR2010/005785
International Filing Date 27.08.2010
IPC
G01N 33/68 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
G01N 33/50 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
CPC
C12Q 1/6837
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6813Hybridisation assays
6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
6837using probe arrays or probe chips
Applicants
  • 한국생명공학연구원 KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY [KR]/[KR] (AllExceptUS)
  • 정봉현 CHUNG, Bong Hyun [KR]/[KR] (UsOnly)
  • 김상규 KIM, Sang Kyu [KR]/[KR] (UsOnly)
  • 조현민 CHO, Hyun Min [KR]/[KR] (UsOnly)
Inventors
  • 정봉현 CHUNG, Bong Hyun
  • 김상규 KIM, Sang Kyu
  • 조현민 CHO, Hyun Min
Agents
  • 손민 SON, Min
Priority Data
10-2009-008509109.09.2009KR
Publication Language Korean (KO)
Filing Language Korean (KO)
Designated States
Title
(EN) METHOD FOR DETECTING AND QUANTIFYING A TARGET SUBSTANCE USING A BIOCHIP
(FR) PROCÉDÉ POUR DÉTECTER ET QUANTIFIER UNE SUBSTANCE CIBLE À L'AIDE D'UNE BIOPUCE
(KO) 바이오칩을 이용한 표적 물질 검출 및 정량 방법
Abstract
(EN)
The present invention relates to a method for detecting and quantifying a target substance using a biochip having a substrate onto which probe molecules are fixed, and more particularly, to a method which involves preparing a biochip having a substrate onto which probe molecules are fixed, and enabling the biochip to contact a specimen containing a target substance having electric charges, conducting a reaction between the target substance and nanoparticles having electric charges that are opposite to those of the target substance, and conducting a reaction with a metal enhancing solution such that the sizes of the nanoparticles are amplified and the target substance can be detected and quantified using the biochip which can be seen by the naked eye.
(FR)
La présente invention porte sur un procédé pour détecter et quantifier une substance cible à l'aide d'une biopuce comportant un substrat sur lequel des molécules de sonde sont fixées, et, plus particulièrement, sur un procédé qui comprend la préparation d'une biopuce comportant un substrat sur lequel des molécules de sonde sont fixées, et le fait de permettre à la biopuce de venir en contact avec un spécimen contenant une substance cible comportant des charges électriques, la réalisation d'une réaction entre la substance cible et des nanoparticules comportant des charges électriques qui sont opposées à celles de la substance cible, et la réalisation d'une réaction avec une solution de renforcement de métal de telle sorte que les tailles des nanoparticules sont amplifiées et que la substance cible peut être détectée et quantifiée à l'aide de la biopuce visible à l'œil nu.
(KO)
본 발명은 기판 상에 프로브 분자가 고정된 바이오칩을 이용하여, 표적 물질을 검출하는 방법 및 정량 방법에 관한 것으로서, 보다 상세하게는 기판 상에 프로브 분자가 고정된 바이오칩을 준비하고, 이를 전하를 갖는 표적 물질을 포함하는 시료와 접촉시킨 후, 상기 표적 물질과 반대 전하를 갖는 나노 입자를 반응시킨 후 금속 인핸싱 용액 (enhancing solution)을 반응시켜서 상기 나노 입자의 크기를 증폭하여 육안으로 바이오칩을 이용한 표적 물질의 검출 방법 및 정량 방법에 관한 것이다.
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