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1. (WO2011021657) METHOD FOR QUANTIFICATION OF L-LYSINE IN BIOLOGICAL SAMPLE
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2011/021657    International Application No.:    PCT/JP2010/063971
Publication Date: 24.02.2011 International Filing Date: 19.08.2010
IPC:
G01N 27/416 (2006.01), C12M 1/40 (2006.01), C12Q 1/26 (2006.01), C12Q 1/28 (2006.01), C12Q 1/32 (2006.01), G01N 27/327 (2006.01), G01N 33/68 (2006.01)
Applicants: TOYAMA PREFECTURE [JP/JP]; 1-7, Shinsogawa, Toyama-shi, Toyama 9308501 (JP) (For All Designated States Except US).
AJINOMOTO CO., INC. [JP/JP]; 15-1, Kyobashi 1-chome, Chuo-ku, Tokyo 1048315 (JP) (For All Designated States Except US).
ASANO, Yasuhisa [JP/JP]; (JP) (For US Only).
MATSUDA, Motoki [JP/JP]; (JP) (For US Only)
Inventors: ASANO, Yasuhisa; (JP).
MATSUDA, Motoki; (JP)
Agent: SIKs & Co.; 8th Floor, Kyobashi-Nisshoku Bldg., 8-7, Kyobashi 1-chome, Chuo-ku Tokyo 1040031 (JP)
Priority Data:
2009-191525 21.08.2009 JP
Title (EN) METHOD FOR QUANTIFICATION OF L-LYSINE IN BIOLOGICAL SAMPLE
(FR) PROCÉDÉ DE QUANTIFICATION DE L-LYSINE DANS UN ÉCHANTILLON BIOLOGIQUE
(JA) 生体試料中のL-リジンの定量法
Abstract: front page image
(EN)Disclosed are: an enzymatic quantification method which can quantify L-lysine in a biological sample specifically even when other amino acid co-exists in the biological sample; and a measurement kit and an enzyme sensor, both of which can be utilized in the practice of the enzymatic quantification method. Specifically disclosed are: a method for measuring L-lysine contained in a biological sample, which comprises allowing an L-lysine ε-oxidase to act on an analyte containing the biological sample and quantifying a product produced by the action of the L-lysine ε-oxidase; an enzyme sensor comprising an L-lysine ε-oxidase; and a kit for measuring L-lysine, which comprises the following reagents: (1) an L-lysine ε-oxidase; and (2) a reagent capable of detecting a product produced by the action of the L-lysine ε-oxidase.
(FR)L'invention concerne un procédé de quantification enzymatique permettant de quantifier spécifiquement L-lysine dans un échantillon biologique, même si d'autres amino-acides sont également présents, ainsi qu'un capteur enzymatique et un kit de mesure pouvant être utilisés lors de la mise en oeuvre de ce procédé de quantification enzymatique. Plus spécifiquement, l'invention concerne un procédé de mesure de L-lysine contenue dans un échantillon biologique, comprenant la mise en réaction de L-lysineε-oxydase dans un spécimen contenant l'échantillon biologique, et la quantification du produit ainsi obtenu, ainsi qu'un capteur enzymatique mettant en oeuvre L-lysineε-oxydase. L'invention concerne encore un kit de mesure de L-lysine comprenant les réactifs suivants: (1) L-lysineε-oxydase (2) un réactif pour la détection d'un produit généré par L-lysineε-oxydase.
(JA)本発明は、生体試料中のL-リジンを、他のアミノ酸が共存する場合であっても特異的に定量可能である酵素的定量法、この酵素的定量法を実施する際に利用できる測定用のキット及び酵素センサーを提供する。本発明は、生体試料を含有する検体に、L-リジンε-オキシダーゼを作用させ、生じた生成物を定量することを含む、前記生体試料に含有されるL-リジンの測定方法、L-リジンε-オキシダーゼを用いる酵素センサーに関する。以下の試薬を含むL-リジンの測定用キットに関する。 (1)L-リジンε-オキシダーゼ (2)L-リジンε-オキシダーゼにより生成する生成物検出用試薬
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)