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1. WO2011009975 - METHOD FOR DETECTING AND IDENTIFYING SPECIES OF THE FAMILY ENGRAULIDAE

Publication Number WO/2011/009975
Publication Date 27.01.2011
International Application No. PCT/ES2010/000314
International Filing Date 19.07.2010
IPC
C12Q 1/68 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
C12N 15/11 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
CPC
C12N 15/11
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
C12Q 1/6888
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
6888for detection or identification of organisms
C12Q 2600/156
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
2600Oligonucleotides characterized by their use
156Polymorphic or mutational markers
Applicants
  • UNIVERSIDAD DE CÁDIZ [ES]/[ES] (AllExceptUS)
  • REBORDINOS GONZÁLEZ, Laureana [ES]/[ES] (UsOnly)
  • CHAIRI, Hicham [ES]/[ES] (UsOnly)
Inventors
  • REBORDINOS GONZÁLEZ, Laureana
  • CHAIRI, Hicham
Priority Data
P20090164922.07.2009ES
Publication Language Spanish (ES)
Filing Language Spanish (ES)
Designated States
Title
(EN) METHOD FOR DETECTING AND IDENTIFYING SPECIES OF THE FAMILY ENGRAULIDAE
(ES) MÉTODO PARA LA DETECCIÓN E IDENTIFICACIÓN DE ESPECIES DE LA FAMILIA ENGRAULIDAE
(FR) PROCÉDÉ POUR LA DÉTECTION ET L'IDENTIFICATION D'ESPÈCES DE LA FAMILLE ENGRAULIDAE
Abstract
(EN)
Method for detecting and identifying species of the family Engraulidae. The present invention relates to a method for the simultaneous detection and identification in a sample of at least one of the species of the family Engraulidae selected from Engraulidis encrasicolus, Engraulis mordax and Engraulis ringens, by multiplex PCR amplification of at least one of the sequences selected from SEQ ID NO 1, SEQ ID NO 2 and SEQ ID NO 3, corresponding to the non-transcribed spacer (NTS) of the 5S ribosomal DNA of at least one of the 3 species, using at least one of the pairs of sequence primers selected from: SEQ ID NO 4 and SEQ ID NO 5; SEQ ID NO 6 and SEQ ID NO 7; and SEQ ID NO 8 and SEQ ID NO 9.
(ES)
Método para la detección e identificación de especies de la familia engraulidae. La presente invención se refiere a un método para la detección e identificación simultánea en una muestra de al menos una de las especies de la familia Engraulidae seleccionada entre Engraulis encrasicolus, Engraulis mordax y Engraulis ringens, mediante amplificación por PCR multiplex de al menos una de la secuencias, seleccionada de entre SEQ ID NO 1, SEQ ID NO 2 y SEQ ID NO 3, correspondiente con el espaciador no transcrito (NTS) del ADN ribosómico 5S de al menos una de las 3 especies, utilizando al menos uno de los pares de cebadores de secuencia seleccionados entre: SEQ ID NO 4 y SEQ ID NO 5; SEQ ID NO 6 y SEQ ID NO 7; y SEQ ID NO 8 y SEQ ID NO 9.
(FR)
L'invention concerne un procédé pour la détection et l'identification d'espèces de la famille engraulidae. Plus précisément, l'invention concerne un procédé pour la détection et l'identification simultanée dans un échantillon d'au moins l'une des espèces de la famille engraulidae sélectionnée parmi Engraulis encrasicolus, Engraulis mordax et Engraulis ringens, par amplification PCR multiplex d'au moins une des séquences sélectionnées parmi SEQ ID NO 1, SEQ ID NO 2 et SEQ ID NO 3, correspondant à l'espaceur non transcrit de l'ADN ribosomique 5S d'au moins une des trois espèces, au moyen d'au moins une des paires d'amorces de séquence sélectionnées parmi: SEQ ID NO 4 et SEQ ID NO 5; SEQ ID NO 6 et SEQ ID NO 7; et SEQ ID NO 8 et SEQ ID NO 9.
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