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1. WO2011008831 - MASS TAGS FOR SPECTROMETRIC ANALYSIS OF IMMUNOGLOBULINS

Publication Number WO/2011/008831
Publication Date 20.01.2011
International Application No. PCT/US2010/041936
International Filing Date 14.07.2010
IPC
G01N 33/53 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
G01N 33/532 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
531Production of immunochemical test materials
532Production of labelled immunochemicals
H01J 49/26 2006.01
HELECTRICITY
01BASIC ELECTRIC ELEMENTS
JELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
49Particle spectrometers or separator tubes
26Mass spectrometers or separator tubes
CPC
G01N 2458/15
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2458Labels used in chemical analysis of biological material
15Non-radioactive isotope labels, e.g. for detection by mass spectrometry
G01N 33/6848
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
6803General methods of protein analysis not limited to specific proteins or families of proteins
6848Methods of protein analysis involving mass spectrometry
H01J 49/00
HELECTRICITY
01BASIC ELECTRIC ELEMENTS
JELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
49Particle spectrometers or separator tubes
Applicants
  • UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC. [US]/[US] (AllExceptUS)
  • YOST, Richard, A. [US]/[US] (UsOnly)
Inventors
  • YOST, Richard, A.
Agents
  • VAN DYKE, Timothy, H.
Priority Data
61/225,27314.07.2009US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) MASS TAGS FOR SPECTROMETRIC ANALYSIS OF IMMUNOGLOBULINS
(FR) MARQUEURS DE MASSE POUR L'ANALYSE SPECTROMÉTRIQUE D'IMMUNOGLOBULINES
Abstract
(EN)
The present invention is a method for the characterization or detection of one or more antibodies in a sample. The method comprises obtaining a tagged antigen comprising an antigen and a mass tag attached to the antigen. The tagged antigen has specificity for the antibody. In addition, the method comprises combining the tagged antigen with the antibody to form a tagged antigen-antibody complex. Further, the method comprises cleaving the mass tag from the tagged antigen-antibody complex. Thereafter, the method comprises analyzing the mass tag via a mass spectrometer to determine the presence of the mass tag in the sample and correlating the presence of the mass tag with a presence of the antibody in the sample.
(FR)
La présente invention a pour objet un procédé pour la caractérisation ou la détection d'un ou plusieurs anticorps dans un échantillon. Le procédé comprend l'obtention d'un antigène marqué comprenant un antigène et un marqueur de masse fixé sur l'antigène. L'antigène marqué possède une spécificité pour l'anticorps. De plus, le procédé comprend la combinaison de l'antigène marqué avec l'anticorps pour former un complexe antigène marqué - anticorps. En outre, le procédé comprend le clivage du marqueur de masse du complexe antigène marqué - anticorps. Par la suite, le procédé comprend l'analyse du marqueur de masse par l'intermédiaire d'un spectromètre de masse pour déterminer la présence du marqueur de masse dans l'échantillon et la corrélation de la présence du marqueur de masse à une présence de l'anticorps dans l'échantillon.
Also published as
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