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1. WO2011008785 - A METHOD OF ANALYZING CELLULOSE DECAY IN CELLULOSIC MATERIAL HYDROLYSIS

Publication Number WO/2011/008785
Publication Date 20.01.2011
International Application No. PCT/US2010/041869
International Filing Date 13.07.2010
IPC
G01N 33/50 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
CPC
C12Q 1/34
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
34involving hydrolase
G01N 2333/924
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2333Assays involving biological materials from specific organisms or of a specific nature
90Enzymes; Proenzymes
914Hydrolases (3)
924acting on glycosyl compounds (3.2)
Applicants
  • NOVOZYMES A/S [DK]/[DK] (AllExceptUS)
  • MALTEN, Marco [DE]/[DK] (UsOnly)
  • MCFARLAND, Keith [US]/[US] (UsOnly)
Inventors
  • MALTEN, Marco
  • MCFARLAND, Keith
Agents
  • STARNES, Robert
Priority Data
61/226,53817.07.2009US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) A METHOD OF ANALYZING CELLULOSE DECAY IN CELLULOSIC MATERIAL HYDROLYSIS
(FR) PROCÉDÉ D'ANALYSE DE DÉTÉRIORATION DE CELLULOSE DANS UNE HYDROLYSE DE MATÉRIAU CELLULOSIQUE
Abstract
(EN) Enzymes and/or polypeptides and/or mixtures of interest are evaluated during hydrolysis of cellulosic material by the use of indicator constituents such as fluorescent agents, resulting in efficient high-throughput analysis of enzymes and/or polypeptides. A high-throughput assay for the analysis of, inter alia, pretreated corn stover (PCS) hydrolysis is also disclosed.
(FR) Selon l'invention, on évalue des enzymes et/ou des polypeptides et/ou des mélanges présentant de l'intérêt lors de l'hydrolyse d'un matériau cellulosique par utilisation de constituants indicateurs tels que des agents fluorescents, ce qui a pour conséquence une analyse efficace à haut rendement des enzymes et/ou des polypeptides. L'invention porte également sur un essai à haut rendement pour l'analyse, entre autres, de l'hydrolyse de canne de maïs prétraité (PCS).
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