Processing

Please wait...

Settings

Settings

Goto Application

1. WO2011008518 - METHODS AND APPARATUS FOR THE DETECTION AND DIFFERENTIATION OF NON-SIALATED PROTEINS FROM SIALATED PROTEINS IN A FLUID SAMPLE

Publication Number WO/2011/008518
Publication Date 20.01.2011
International Application No. PCT/US2010/040305
International Filing Date 29.06.2010
IPC
G01N 33/53 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
G01N 33/52 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
52Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper
CPC
B01L 2200/10
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2200Solutions for specific problems relating to chemical or physical laboratory apparatus
10Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
B01L 2300/0681
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2300Additional constructional details
06Auxiliary integrated devices, integrated components
0681Filter
B01L 2300/087
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2300Additional constructional details
08Geometry, shape and general structure
0861Configuration of multiple channels and/or chambers in a single devices
087Multiple sequential chambers
B01L 3/502707
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
3Containers or dishes for laboratory use, e.g. laboratory glassware
50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
502with fluid transport, e.g. in multi-compartment structures
5027by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
502707characterised by the manufacture of the container or its components
G01N 2333/79
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2333Assays involving biological materials from specific organisms or of a specific nature
435from animals; from humans
79Transferrins, e.g. lactoferrins, ovotransferrins
G01N 33/6893
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
6893related to diseases not provided for elsewhere
Applicants
  • COLEN INNOVATIONS, LLC [US]/[US] (AllExceptUS)
  • COLEN, Chaim, BenJoseph [US]/[US] (UsOnly)
Inventors
  • COLEN, Chaim, BenJoseph
Agents
  • DAVIS, Paul
Priority Data
12/824,82728.06.2010US
61/225,52314.07.2009US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHODS AND APPARATUS FOR THE DETECTION AND DIFFERENTIATION OF NON-SIALATED PROTEINS FROM SIALATED PROTEINS IN A FLUID SAMPLE
(FR) PROCÉDÉS ET APPAREIL POUR LA DÉTECTION ET LA DIFFÉRENCIATION DE PROTÉINES NON SIALISÉES À PARTIR DE PROTÉINES SIALISÉES DANS UN ÉCHANTILLON DE FLUIDE
Abstract
(EN)
The present invention is directed to methods and devices for detection of cerebrospinal fluid leaks by detection of the CSF protein beta-2 transferrin. The micro fluidic devices and methods of the invention combine capture and specific labeling of transferrin from a sample with a subsequent step of isoelectric focusing to separate transferrin isoforms for detection. Micro fluidic channels and chambers are patterned on a substrate, designed so that on one region (i.e., a microfluidic channel or chamber) of the substrate transferrin is selectively captured from the sample and labeled, and in a second region of the susbstrate, transferrin isoforms are separated using isoelectric focusing. Detection of two transferrin bands, indicating the presence of beta-2-transferrin, indicates the presence of CSF in the sample. The devices and methods of the invention provide a safe, efficient, and ultrarapid modality with high specificity and sensitivity for the detection of CSF in the acute care setting.
(FR)
La présente invention porte sur des procédés et des dispositifs pour la détection de fuites de fluide cérébrospinal (CSF) par la détection de la protéine du CSF bêta-2 transférine. Les dispositifs et procédés microfluidiques de l'invention combinent la capture et le marquage spécifiques de la transferrine à partir d'un échantillon avec une étape ultérieure de focalisation isoélectrique pour séparer des isoformes de transferrine pour une détection. Des canaux et des chambres microfluidiques sont formés sur un substrat, conçus de telle sorte qu'une région (à savoir, un canal ou une chambre microfluidique) de la transferrine de substrat est sélectivement capturée à partir de l'échantillon et marquée, et dans une seconde région du substrat, des isoformes de transferrine sont séparés à l'aide d'une focalisation isoélectrique. La détection de deux bandes de transferrine, indiquant la présence de la bêta-2-transferrine, indique la présence de CSF dans l'échantillon. Les dispositifs et procédés de l'invention fournissent une modalité sûre, efficace et ultrarapide avec une spécificité et une sensibilité élevées pour la détection de CSF dans le cadre de soins actifs.
Latest bibliographic data on file with the International Bureau