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1. WO2011001906 - METHOD FOR EVALUATION OF CULTURED CELLS, AND METHOD FOR SCREENING OF BIOMARKER

Publication Number WO/2011/001906
Publication Date 06.01.2011
International Application No. PCT/JP2010/060812
International Filing Date 25.06.2010
IPC
C12Q 1/68 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
C12N 15/09 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
CPC
C12Q 1/6881
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
6881for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
C12Q 1/6883
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
6883for diseases caused by alterations of genetic material
C12Q 1/6886
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
6883for diseases caused by alterations of genetic material
6886for cancer
C12Q 2600/118
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
2600Oligonucleotides characterized by their use
118Prognosis of disease development
C12Q 2600/156
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
2600Oligonucleotides characterized by their use
156Polymorphic or mutational markers
C12Q 2600/158
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
2600Oligonucleotides characterized by their use
158Expression markers
Applicants
  • 富士レビオ株式会社 FUJIREBIO INC. [JP]/[JP] (AllExceptUS)
  • 堀田 佳之 HOTTA, Yoshiyuki [JP]/[JP] (UsOnly)
  • 小見 和也 OMI, Kazuya [JP]/[JP] (UsOnly)
  • 岡 麻子 OKA, Asako [JP]/[JP] (UsOnly)
Inventors
  • 堀田 佳之 HOTTA, Yoshiyuki
  • 小見 和也 OMI, Kazuya
  • 岡 麻子 OKA, Asako
Agents
  • 谷川 英次郎 TANIGAWA, Hidejiro
Priority Data
2009-15444930.06.2009JP
Publication Language Japanese (JA)
Filing Language Japanese (JA)
Designated States
Title
(EN) METHOD FOR EVALUATION OF CULTURED CELLS, AND METHOD FOR SCREENING OF BIOMARKER
(FR) PROCÉDÉ D'ÉVALUATION DE CELLULES CULTIVÉES ET PROCÉDÉ DE CRIBLAGE DE BIOMARQUEURS
(JA) 培養細胞の評価方法およびバイオマーカーのスクリーニング方法
Abstract
(EN)
Disclosed is a novel means for evaluating cultured cells without consuming the cultured cells or for screening for a biomarker. Specifically disclosed are: a method for evaluating cultured cells, which comprises culturing cells in a serum-free culture medium and measuring at least one of nucleic acids related from the cells into a culture; and a method for screening for a biomarker, which comprises culturing cells in a serum-free culture medium and measuring nucleic acids released from the cells into a culture. For example, the nucleic acid to be employed as a measure is micro-RNA.
(FR)
La présente invention concerne un moyen inédit d'évaluation de cellules cultivées sans destruction de celles-ci ou, encore, un moyen inédit de criblage à la recherche d'un biomarqueur. L'invention concerne, plus précisément, un procédé d'évaluation de cellules cultivées, comprenant la culture de cellules dans un milieu de culture dépourvu de sérum et le dosage, dans une culture, d'au moins un acide nucléique associé, issu des cellules; et un procédé de criblage à la recherche d'un biomarqueur, comprenant la culture de cellules dans un milieu de culture dépourvu de sérum et le dosage des acides nucléiques libérés par les cellules dans une culture. L'acide nucléique devant être utilisé dans le cadre du dosage peut, par exemple, être un micro-ARN.
(JA)
培養細胞を消費することなく培養細胞の評価やバイオマーカーのスクリーニングをすることができる新規な手段が開示されている。本発明の培養細胞の評価方法は、無血清培地中で細胞を培養し、該細胞から培養液中に放出される核酸の少なくとも1種を測定することを含む。また、本発明のバイオマーカーのスクリーニング方法は、無血清培地中で細胞を培養し、該細胞から培養液中に放出される核酸を測定することを含む。指標とする核酸は、例えばマイクロRNAである。
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