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1. WO2011001045 - ANALYSIS AND ASSAY OF GLYCATED HEMOGLOBINS BY CAPILLARY ELECTROPHORESIS, BUFFER COMPOSITIONS, AND KITS FOR CAPILLARY ELECTROPHORESIS

Publication Number WO/2011/001045
Publication Date 06.01.2011
International Application No. PCT/FR2010/000481
International Filing Date 30.06.2010
IPC
G01N 27/447 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
27Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
26by investigating electrochemical variables; by using electrolysis or electrophoresis
416Systems
447using electrophoresis
CPC
G01N 27/447
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
27Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
26by investigating electrochemical variables; by using electrolysis or electrophoresis
416Systems
447using electrophoresis
G01N 27/44747
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
27Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
26by investigating electrochemical variables; by using electrolysis or electrophoresis
416Systems
447using electrophoresis
44704Details; Accessories
44747Composition of gel or of carrier mixture
G01N 27/44791
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
27Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
26by investigating electrochemical variables; by using electrolysis or electrophoresis
416Systems
447using electrophoresis
44756Apparatus specially adapted therefor
44791Microapparatus
G01N 33/72
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
72involving blood pigments, e.g. haemoglobin, bilirubin ; or other porphyrins; involving occult blood
G01N 33/723
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
72involving blood pigments, e.g. haemoglobin, bilirubin ; or other porphyrins; involving occult blood
721Haemoglobin
723Glycosylated haemoglobin
Applicants
  • SEBIA [FR]/[FR] (AllExceptUS)
  • DESCHAMPS, Gérald [FR]/[FR] (UsOnly)
  • ROBERT, Frédéric [FR]/[FR] (UsOnly)
  • SIMONIN, Denis [FR]/[FR] (UsOnly)
Inventors
  • DESCHAMPS, Gérald
  • ROBERT, Frédéric
  • SIMONIN, Denis
Agents
  • DESAIX, Anne
Priority Data
09/0322001.07.2009FR
Publication Language French (FR)
Filing Language French (FR)
Designated States
Title
(EN) ANALYSIS AND ASSAY OF GLYCATED HEMOGLOBINS BY CAPILLARY ELECTROPHORESIS, BUFFER COMPOSITIONS, AND KITS FOR CAPILLARY ELECTROPHORESIS
(FR) ANALYSE ET DOSAGE D'HÉMOGLOBINES GLYQUÉES PAR ÉLECTROPHORÈSE CAPILLAIRE, COMPOSITIONS TAMPON ET KITS POUR ÉLECTROPHORÈSE CAPILLAIRE
Abstract
(EN)
The invention relates to a method for the analysis of glycated hemoglobins by capillary electrophoresis, said glycated hemoglobins comprising at least one globin chain including a glucose residue bonded to the amino acid in the N-terminal position and being contained in a biological sample, wherein said method includes the implementation of a buffer composition including at least one compound capable of specifically complexing glucose residues of one or more glycated hemoglobins and conveying, to said hemoglobin(s), a plurality negative electric charges having an alkaline pH. For example, said compound can be 3,4- or 3,5-dicarboxyphenylboronic acid, preferably 3,5-dicarboxyphenylboronic acid. Said method can particularly be used for separating and assaying the HbAi0 hemoglobin present in a biological sample optionally comprising other hemoglobins, in particular other minor fractions. The invention also relates to buffer compositions useful for said analysis, as well as to kits for analyzing and assaying glycated hemoglobins by capillary electrophoresis.
(FR)
L'invention concerne une méthode d'analyse par électrophorèse capillaire d'hémoglobines glyquées comportant au moins une chaîne de globine comportant un résidu glucose lié à l'acide aminé se trouvant en position N-terminale, contenues dans un échantillon biologique, ladite méthode comprenant la mise en œuvre d'une composition tampon comprenant au moins un composé capable de complexer spécifiquement des résidus glucose d'une ou plusieurs hémoglobine(s) glyquée(s) et d'apporter à cette ou ces hémoglobine(s) glyquée(s) plusieurs charges électriques négatives à pH alcalin. A titre d'exemple, ce composé peut être l'acide 3,4- ou 3.5- diCarboxyPhenylBoronique, de préférence l'acide 3,5- diCarboxyPhenylBoronique. Ladite méthode peut notamment être utilisée pour séparer et doser l'hémoglobine HbAi0 présente dans un échantillon biologique comportant éventuellement d'autres hémoglobines, en particulier d'autres fractions mineures. L'invention concerne également des compositions tampon utiles pour cette analyse, ainsi que des trousses d'analyse et de dosage d'hémoglobines glyquées par électrophorèse capillaire.
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