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1. WO2011000933 - ENHANCED PHYTASE VARIANTS

Publication Number WO/2011/000933
Publication Date 06.01.2011
International Application No. PCT/EP2010/059413
International Filing Date 01.07.2010
IPC
C12N 9/16 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14Hydrolases (3.)
16acting on ester bonds (3.1)
C12N 15/55 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
52Genes encoding for enzymes or proenzymes
55Hydrolases (3)
A23K 1/16 2006.01
AHUMAN NECESSITIES
23FOODS OR FOODSTUFFS; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES
KFEEDING-STUFFS SPECIALLY ADAPTED FOR ANIMALS; METHODS SPECIALLY ADAPTED FOR PRODUCTION THEREOF
1Animal feeding-stuffs
16supplemented with accessory food factors; Salt blocks
A23K 1/165 2006.01
AHUMAN NECESSITIES
23FOODS OR FOODSTUFFS; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES
KFEEDING-STUFFS SPECIALLY ADAPTED FOR ANIMALS; METHODS SPECIALLY ADAPTED FOR PRODUCTION THEREOF
1Animal feeding-stuffs
16supplemented with accessory food factors; Salt blocks
165with steroids, hormones, or enzymes
CPC
C12N 9/16
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
14Hydrolases (3)
16acting on ester bonds (3.1)
C12Y 301/03008
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
YENZYMES
301Hydrolases acting on ester bonds (3.1)
03Phosphoric monoester hydrolases (3.1.3)
030083-Phytase (3.1.3.8)
C12Y 301/03026
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
YENZYMES
301Hydrolases acting on ester bonds (3.1)
03Phosphoric monoester hydrolases (3.1.3)
030264-Phytase (3.1.3.26), i.e. 6-phytase
Applicants
  • BIOMETHODES [FR]/[FR] (AllExceptUS)
  • BLESA, Stéphane [FR]/[FR] (UsOnly)
  • CHAUTARD, Hélène [FR]/[FR] (UsOnly)
  • DELCOURT, Marc [FR]/[FR] (UsOnly)
  • MESTA, Laurent [FR]/[FR] (UsOnly)
  • WINTER, Bruno [FR]/[DE] (UsOnly)
Inventors
  • BLESA, Stéphane
  • CHAUTARD, Hélène
  • DELCOURT, Marc
  • MESTA, Laurent
  • WINTER, Bruno
Agents
  • THOMAS, Nadine
Priority Data
095449001.07.2009FR
Publication Language French (FR)
Filing Language French (FR)
Designated States
Title
(EN) ENHANCED PHYTASE VARIANTS
(FR) VARIANTS AMELIORES D'UNE PHYTASE
Abstract
(EN)
The present invention relates to the field of enhancing proteins, and in particular to the field of proteins enhanced by molecular evolution. The invention relates to a so-called enhanced phytase variant, characterised in that said phytase has higher thermostability and/or activity than the original phytase. The invention also relates to an encoding nucleic acid for said variant, to a cassette or an expression vector containing said variant, to a host cell expressing said variant, to a composition including said variant, as well as to the uses thereof, mainly for preparing food additives and for feeding animals.
(FR)
La présente invention relève du domaine de l'amélioration des protéines et en particulier celui des protéines améliorées par évolution moléculaire. Elle porte sur un variant d'une phytase, dit amélioré, en ce qu'il présente une thermostabilité et / ou une activité supérieures à la phytase d'origine. L'invention porte également sur un acide nucléique codant pour ce variant, une cassette ou un vecteur d'expression contenant ce variant, une cellule hôte exprimant ce variant, une composition comprenant ce variant ainsi que les utilisations de ceux-ci principalement dans la préparation d'additifs alimentaires et l'alimentation animale.
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