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1. (WO2010151688) CAPTURE PURIFICATION PROCESSES FOR PROTEINS EXPRESSED IN A NON-MAMMALIAN SYSTEM
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2010/151688    International Application No.:    PCT/US2010/039854
Publication Date: 29.12.2010 International Filing Date: 24.06.2010
IPC:
C07K 1/22 (2006.01), C07K 1/32 (2006.01), C07K 1/18 (2006.01)
Applicants: AMGEN INC. [US/US]; One Amgen Center Drive Thousand Oaks, CA 91320-1799 (US) (For All Designated States Except US).
SHULTZ, Joseph, Edward [US/US]; (US) (For US Only).
HART, Roger [US/US]; (US) (For US Only)
Inventors: SHULTZ, Joseph, Edward; (US).
HART, Roger; (US)
Agent: LAMERDIN, John, A.; Amgen Inc. One Amgen Center Drive Patent Operations, M/S 28-2-C Thousand Oaks, CA 91320-1799 (US)
Priority Data:
61/220,477 25.06.2009 US
Title (EN) CAPTURE PURIFICATION PROCESSES FOR PROTEINS EXPRESSED IN A NON-MAMMALIAN SYSTEM
(FR) PROCÉDÉS DE PURIFICATION PAR CAPTURE DE PROTÉINES EXPRIMÉES DANS UN SYSTÈME NON MAMMIFÈRE
Abstract: front page image
(EN)Methods of purifying proteins expressed in non-mammalian expression systems in a non-native soluble form directly from cell lysate are disclosed. Methods of purifying proteins expressed in non-mammalian expression systems in a non-native limited solubility form directly from a refold solution are also disclosed. Resin regeneration methods are also provided.
(FR)L'invention concerne des procédés de purification de protéines exprimées dans des systèmes d'expression non mammifères sous une forme soluble non native directement à partir d'un lysat cellulaire. L'invention concerne également des procédés de purification de protéines exprimées dans des systèmes d'expression non mammifères sous une forme à solubilité limitée non native directement à partir d'une solution de repliement. L'invention concerne également des procédés de régénération de résine.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)