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1. (WO2010147904) IMPROVED OPTIMIZED STRAINS OF YARROWIA LIPOLYTICA FOR HIGH EICOSAPENTAENOIC ACID PRODUCTION
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2010/147904    International Application No.:    PCT/US2010/038535
Publication Date: 23.12.2010 International Filing Date: 14.06.2010
IPC:
C07K 14/195 (2006.01)
Applicants: E. I. DU PONT DE NEMOURS AND COMPANY [US/US]; 1007 Market Street Wilmington, Delaware 19898 (US) (For All Designated States Except US).
HONG, Seung-Pyo [AU/US]; (US) (For US Only).
SHARPE, Pamela, L. [US/US]; (US) (For US Only).
XUE, Zhixiong [CN/US]; (US) (For US Only).
YADAV, Narendra, S. [US/US]; (US) (For US Only).
ZHU, Qun [US/US]; (US) (For US Only)
Inventors: HONG, Seung-Pyo; (US).
SHARPE, Pamela, L.; (US).
XUE, Zhixiong; (US).
YADAV, Narendra, S.; (US).
ZHU, Qun; (US)
Agent: CHRISTENBURY, Lynne, M.; E. I. du Pont de Nemours and Company Legal Patent Records Center 4417 Lancaster Pike Wilmington, Delaware 19805 (US)
Priority Data:
61/187,366 16.06.2009 US
Title (EN) IMPROVED OPTIMIZED STRAINS OF YARROWIA LIPOLYTICA FOR HIGH EICOSAPENTAENOIC ACID PRODUCTION
(FR) SOUCHES OPTIMISÉES AMÉLIORÉES DE YARROWIA LIPOLYTICA POUR UNE PRODUCTION ÉLEVÉE D'ACIDE ÉICOSAPENTAÉNOÏQUE
Abstract: front page image
(EN)Described are engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing an oil comprising greater than 50 weight percent of eicosapentaenoic acid ["EPA"], an ω-3 polyunsaturated fatty acid, measured as a weight percent of total fatty acids ["% TFAs"] and having a ratio of at least 3.1 of EPA % TFAs, to linoleic acid, measured as % TFAs. These strains over-express at least one Δ9 elongase/Δ8 desaturase multizyme, in addition to other heterologous Δ9 elongases, Δ8 desaturases, Δ5 desaturases, Δ17 desaturases, Δ12 desaturases, C16/18 elongases, and optionally over-express diacylglycerol cholinephosphotransferases, malonyl CoA synthetases and/or acyl-CoA lysophospholipid acyltransferases. The expression of at least one peroxisome biogenesis factor protein is down-regulated. Methods for producing EPA within said host cells, oils obtained from the cells, and products therefrom are claimed.
(FR)L'invention porte sur des souches modifiées par génie génétique de la levure oléagineuse Yarrowia lipolytica capable de produire une huile comprenant plus de 50 pour cent en poids d'acide éicosapentaénoïque [« EPA »], un acide gras ω-3 polyinsaturé, mesuré en pourcentage en poids des acides gras totaux [« % de TFA »] et ayant un rapport d'au moins 3.1 de EPA % TFA, sur l'acide linoléique, mesuré en tant que % de TFA. Ces souches surexpriment au moins une multizyme Δ9 élongase/Δ8 désaturase, en plus d'autres Δ9 élongases, Δ8 désaturases, Δ5 désaturases, Δ17 désaturases, Δ12 désaturases, C16/18 élongases hétérologues et facultativement surexpriment des diacylglycérol cholinephosphotransférases, des malonyl CoA synthétases et/ou des acyl-CoA lysophospholipide acyltransférases. L'expression d'au moins une protéine de facteur de biogenèse de peroxisome est régulée de manière négative. L'invention porte sur des procédés pour produire de l'EPA dans lesdites cellules hôtes, sur des huiles obtenues à partir des cellules et sur les produits de celles-ci.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)