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1. (WO2010138800) SONICATION CARTRIDGE FOR NUCLEIC ACID EXTRACTION
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2010/138800 International Application No.: PCT/US2010/036546
Publication Date: 02.12.2010 International Filing Date: 28.05.2010
IPC:
C12N 13/00 (2006.01)
Applicants: SADRI, Amir M.[CA/CA]; CA (UsOnly)
KIRCANSKI, Nenad[CA/CA]; CA (UsOnly)
KIRCANSKI, Manja[CA/CA]; CA (UsOnly)
NIKOLIC, Neven[CA/CA]; CA (UsOnly)
TIMOTIJEVIC, Milija[CA/CA]; CA (UsOnly)
BIO-RAD LABORATORIES, INC.[US/US]; 1000 Alfred Nobel Drive Hercules, California 94547, US (AllExceptUS)
Inventors: SADRI, Amir M.; CA
KIRCANSKI, Nenad; CA
KIRCANSKI, Manja; CA
NIKOLIC, Neven; CA
TIMOTIJEVIC, Milija; CA
Agent: HEINES, M. Henry; Townsend and Townsend and Crew LLP Two Embarcadero Center, 8th Floor San Francisco, California 94111-3834, US
Priority Data:
12/788,77727.05.2010US
61/182,18329.05.2009US
Title (EN) SONICATION CARTRIDGE FOR NUCLEIC ACID EXTRACTION
(FR) CARTOUCHE DE SONICATION POUR EXTRACTION D'ACIDE NUCLÉIQUE
Abstract: front page image
(EN) A cartridge in which sonication is applied to biological matter to disrupt and release nucleic acids from the matter is formed from a cartridge body containing a series of wells connected by fluid passages engineered to prevent backflow, with at least one well containing a sonication window covered by a thin lamina to transmit sonic vibrations from a sonication horn contacting the exterior surface of the window. Fluid transport among the wells is achieved by pressure differentials through the fluid passages, and a succession of functions is performed in the various wells, including disruption, mixing, binding of the released nucleic acids to binding materials, washing, elution, and collection.
(FR) L'invention concerne une cartouche destinée à appliquer une sonication à une matière biologique pour interrompre et libérer des acides nucléiques de la matière qui est formée à partir d'un corps de cartouche contenant une série de puits par des passages fluidiques produits par ingéniérie pour empêcher le reflux, un puits au moins contenant une fenêtre de sonication recouverte d'une lame mince permettant de faire transiter des vibrations sonores d'une corne en contact avec la surface extérieure de la fenêtre. Le transport de fluide entre les puits est effectué par des différentiels de pression à travers les passages fluidiques, et une succession de fonctions sont exécutées dans les puits variés, notamment disruption, mélange, liaison d'acides nucléiques libérés à des matériaux de liaison, lavage, élution, et collecte.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)