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1. WO2010136706 - NOVEL METHOD FOR QUANTIFYING PROTEINS BY MASS SPECTROMETRY

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Publication Number WO/2010/136706
Publication Date 02.12.2010
International Application No. PCT/FR2010/050991
International Filing Date 21.05.2010
IPC
G01N 33/68 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
CPC
C07K 1/16
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
1General methods for the preparation of peptides ; , i.e. processes for the organic chemical preparation of peptides or proteins of any length
14Extraction; Separation; Purification
16by chromatography
C12Q 1/37
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
34involving hydrolase
37involving peptidase or proteinase
G01N 33/68
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
G01N 33/6848
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
6803General methods of protein analysis not limited to specific proteins or families of proteins
6848Methods of protein analysis involving mass spectrometry
Applicants
  • BIOMÉRIEUX [FR]/[FR] (AllExceptUS)
  • UNIVERSITE CLAUDE BERNARD LYON I [FR]/[FR] (AllExceptUS)
  • CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE [FR]/[FR] (AllExceptUS)
  • LEMOINE, Jérôme [FR]/[FR] (UsOnly)
  • SALVADOR, Arnaud [FR]/[FR] (UsOnly)
  • CHARRIER, Jean-Philippe [FR]/[FR] (UsOnly)
  • FORTIN, Tanguy [FR]/[FR] (UsOnly)
Inventors
  • LEMOINE, Jérôme
  • SALVADOR, Arnaud
  • CHARRIER, Jean-Philippe
  • FORTIN, Tanguy
Agents
  • SARLIN, Laure
Priority Data
095357629.05.2009FR
Publication Language French (fr)
Filing Language French (FR)
Designated States
Title
(EN) NOVEL METHOD FOR QUANTIFYING PROTEINS BY MASS SPECTROMETRY
(FR) NOUVEAU PROCEDE DE QUANTIFICATION DE PROTEINES PAR SPECTROMETRIE DE MASSE
Abstract
(EN) The present invention relates to a method for the quantitative detection of a target protein in a sample, in which the second-generation fragment ions are detected for providing a series of quantitative measurements, at least one of which is correlated to the amount of proteotypic peptide generated and to the amount of target protein in the sample, characterized in that the selected first-generation fragment ion having a mass (m/z)2 is a doubly-charged peptide having a proline and/or a histidine in position 1.
(FR) La présente invention concerne un procédé pour détecter, de manière quantitative, une protéine cible dans un échantillon, dans lequel des ions fragments de seconde génération sont détectés pour donner une série de mesures quantitatives, dont au moins une est corrélée à la quantité du peptide protéotypique généré et à la quantité de protéine cible présente dans l'échantillon, caractérisé en ce que l'ion fragment de première génération de masse (m/z)2 sélectionné est un peptide doublement chargé qui présente, une proline et/ou une histidine en position 1.
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