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1. (WO2010123014) METHOD FOR PREPARING CELL HIGHLY PRODUCING HETEROGENEOUS PROTEIN
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2010/123014 International Application No.: PCT/JP2010/057030
Publication Date: 28.10.2010 International Filing Date: 21.04.2010
IPC:
C12N 15/09 (2006.01) ,C12N 1/15 (2006.01) ,C12N 1/19 (2006.01) ,C12N 1/21 (2006.01) ,C12N 5/10 (2006.01) ,C12P 21/02 (2006.01) ,C12P 21/08 (2006.01)
Applicants: TABUCHI, Hisahiro[JP/JP]; JP (UsOnly)
SUGIYAMA, Tomoya[JP/JP]; JP (UsOnly)
CHUGAI SEIYAKU KABUSHIKI KAISHA[JP/JP]; 5-1, Ukima 5-chome, Kita-ku, Tokyo 1158543, JP (AllExceptUS)
Inventors: TABUCHI, Hisahiro; JP
SUGIYAMA, Tomoya; JP
Agent: MAYAMA, Setsuko; Nohki-kaikan Fourth Floor, 30-1, Tsuruyacho 3-chome, Kanagawa-ku, Yokohama-shi, Kanagawa 2210835, JP
Priority Data:
2009-10359622.04.2009JP
Title (EN) METHOD FOR PREPARING CELL HIGHLY PRODUCING HETEROGENEOUS PROTEIN
(FR) PROCÉDÉ DE PRÉPARATION D'UNE CELLULE CAPABLE D'UNE FORTE PRODUCTION DE PROTÉINE HÉTÉROGÈNE
(JA) 異種タンパク質を高生産する細胞の作製方法
Abstract: front page image
(EN) Disclosed are a cell capable of highly producing a polypeptide and a method for preparing the cell. A method for preparing a cell capable of highly producing a desired polypeptide, comprising: culturing a cell, which strongly expresses a bicarbonate transporter and carries a DNA encoding the desired polypeptide having been transferred thereinto, in the presence of methotrexate at a high concentration; and selecting a cell, which is capable of highly producing the desired polypeptide, from among surviving cells.
(FR) L'invention porte sur une cellule capable d'une forte production d'un polypeptide et sur un procédé de préparation de la cellule. Le procédé de préparation de la cellule capable d'une forte production d'un polypeptide désiré selon l'invention comprend : la culture d'une cellule, qui exprime fortement un transporteur bicarbonate et porte un ADN codant pour le polypeptide désiré ayant été transféré dans celui-ci, en présence de méthotrexate à une concentration élevée ; et la sélection d'une cellule, qui est capable d'une forte production du polypeptide désiré à partir des cellules survivantes.
(JA)  ポリペプチドを高生産する細胞及びその作製方法を提供する。 Bicarbonateトランスポーターを強発現し、且つ所望のポリペプチドをコードするDNAが導入された細胞を高濃度メトトレキセートの存在下で培養し、生存する細胞から所望のポリペプチドを高産生する細胞を選択することを含む、所望のポリペプチドを高産生する細胞の作製方法。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (EPO) (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)