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1. (WO2010120739) PROTEIN PURIFICATION BY CITRATE PRECIPITATION

Pub. No.:    WO/2010/120739    International Application No.:    PCT/US2010/030841
Publication Date: Fri Oct 22 01:59:59 CEST 2010 International Filing Date: Wed Apr 14 01:59:59 CEST 2010
IPC: C07K 1/30
Applicants: BRISTOL-MYERS SQUIBB COMPANY
ARUNAKUMARI, Alahari
FERREIRA, Gisela Maria Marques
Inventors: ARUNAKUMARI, Alahari
FERREIRA, Gisela Maria Marques
Title: PROTEIN PURIFICATION BY CITRATE PRECIPITATION
Abstract:
The invention provides methods for isolating proteins in purified form from mixtures by precipitation with citrate. The methods are advantageous in that they effectively separate a protein from lower molecular weight contaminants, including fragments or portions of the protein. Such methods are particularly useful for purifying antibodies from mixtures containing antibody proteolytic fragments and unpaired chains.