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1. (WO2010120501) ASSYS FOR BACTERIAL DETECTION AND IDENTIFICATION

Pub. No.:    WO/2010/120501    International Application No.:    PCT/US2010/029314
Publication Date: Fri Oct 22 01:59:59 CEST 2010 International Filing Date: Thu Apr 01 01:59:59 CEST 2010
IPC: G01N 33/569
C07K 16/12
C07K 16/18
C12N 9/52
C12R 1/46
Applicants: SAUREUS, INC.
CHEUNG, Ambrose, Lin-Yau
NEWCOMB, George, Lee
Inventors: CHEUNG, Ambrose, Lin-Yau
NEWCOMB, George, Lee
Title: ASSYS FOR BACTERIAL DETECTION AND IDENTIFICATION
Abstract:
The invention herein generally relates to kits and methods for detecting the presence of a bacterium in a subject, for example, methicillin resistant S. aureus. In certain embodiments, the invention provides a method of detecting presence of a bacterium in a sample from a subject, the method including: contacting a sample from a subject with a bacterium-specific lytic enzyme or lysostaphin capable of specific lysis of a first bacterium if present in the sample, thereby exposing an intracellular gene or gene product of the first bacterium; contacting the sample with a particle having a protein on a surface of the particle in a presence of an antibody in which an Fc portion specifically binds the protein and an F(ab)2 portion specifically binds the intracellular gene or gene product of the first bacterium, with the proviso that when the particle is a second bacterium, the second bacterium is different from the first bacterium; and detecting the presence or absence of the first bacterium by observing the sample for an agglutination reaction, wherein agglutination indicates the presence of the first bacterium in the sample.