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1. (WO2010118865) SYSTEM AND METHOD FOR DETECTION OF HLA VARIANTS

Pub. No.:    WO/2010/118865    International Application No.:    PCT/EP2010/002307
Publication Date: Fri Oct 22 01:59:59 CEST 2010 International Filing Date: Fri Apr 16 01:59:59 CEST 2010
IPC: C12Q 1/68
Applicants: ROCHE DIAGNOSTICS GMBH
F. HOFFMANN-LA ROCHE AG
Inventors: BENTLEY, Gordon
ERLICH, Henry, A.
HIGUCHI, Russell, Gene
HOLCOMB, Cherie
Title: SYSTEM AND METHOD FOR DETECTION OF HLA VARIANTS
Abstract:
A method for detecting one or more HLA sequence types is described that comprises the steps of: amplifying a plurality of first amplicons from a double stranded nucleic acid sample, wherein the first amplicons are amplified with a plurality of pairs of nucleic acid primers that define exons 2 and 3 of both strands of HLA loci from the group consisting of HLA-A, HLA-B, and HLA-C; amplifying the first amplicons to produce a plurality of populations of second amplicons, wherein each population of second amplicons is clonally amplified from one of the first amplicons; sequencing the plurality of populations of second amplicons to generate a nucleic acid sequence composition for each of the plurality of second amplicons; and detecting variation in the sequence composition from one or more of the second amplicons for one or more of the HLA loci..