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1. (WO2010118337) METHODS OF ENHANCING YIELD OF ACTIVE IGA PROTEASE
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2010/118337 International Application No.: PCT/US2010/030562
Publication Date: 14.10.2010 International Filing Date: 09.04.2010
IPC:
C12N 9/52 (2006.01) ,C12N 15/57 (2006.01) ,C12N 15/70 (2006.01) ,C12P 21/02 (2006.01)
Applicants: LONG, Shinong[CN/US]; US (UsOnly)
VELLARD, Michel, Claude[FR/US]; US (UsOnly)
BIOMARIN PHARMACEUTICAL INC.[US/US]; 105 Digital Drive Novato, CA 94949, US (AllExceptUS)
Inventors: LONG, Shinong; US
VELLARD, Michel, Claude; US
Agent: NEVILLE, Katherine, L.; Marshall, Gerstein & Borun LLP 233 S. Wacker Drive 6300 Willis Tower Chicago, IL 60606-6357, US
Priority Data:
61/168,42910.04.2009US
61/234,00414.08.2009US
Title (EN) METHODS OF ENHANCING YIELD OF ACTIVE IGA PROTEASE
(FR) PROCÉDÉS POUR AMÉLIORER LE RENDEMENT EN IGA PROTÉASE ACTIVE
Abstract:
(EN) The present disclosure relates in general to methods for recombinantly producing soluble, active IgA proteases (e.g., IgAl proteases) in host cells (e.g., bacterial cells). In certain embodiments, the present methods involve expression of only a portion of an IgA protease (e.g., only the proteolytic protease domain, and neither the alpha protein domain nor the beta core domain), and provide increased yield of soluble, active IgA protease and increased yield of active IgA protease formed from solubilization and refolding of IgA protease inclusion bodies.
(FR) La présente invention concerne en général des procédés pour produire de manière recombinante des IgA protéases solubles, actives (par exemple, les IgA1 protéases) dans des cellules hôtes (par exemple, des cellules bactériennes), et des procédés pour utiliser les IgA protéases (par exemple, les IgA1 protéases) produites par les procédés dans le traitement de troubles liés au dépôt d'IgA (par exemple, la néphropathie à IgA).
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (EPO) (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)