WIPO logo
Mobile | Deutsch | Español | Français | 日本語 | 한국어 | Português | Русский | 中文 | العربية |
PATENTSCOPE

Search International and National Patent Collections
World Intellectual Property Organization
Options
Query Language
Stem
Sort by:
List Length
Some content of this application is unavailable at the moment.
If this situation persist, please contact us atFeedback&Contact
1. (WO2010116681) METHOD FOR COLLECTING POLYHYDROXYALKANOATE
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2010/116681 International Application No.: PCT/JP2010/002304
Publication Date: 14.10.2010 International Filing Date: 30.03.2010
IPC:
C12P 7/62 (2006.01) ,C08G 63/89 (2006.01) ,C08L 101/16 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7
Preparation of oxygen-containing organic compounds
62
Carboxylic acid esters
C CHEMISTRY; METALLURGY
08
ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
G
MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
63
Macromolecular compounds obtained by reactions forming a carboxylic ester link in the main chain of the macromolecule
88
Post-polymerisation treatment
89
Recovery of the polymer
C CHEMISTRY; METALLURGY
08
ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
L
COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
101
Compositions of unspecified macromolecular compounds
16
the macromolecular compounds being biodegradable
Applicants: YANAGITA, Yoshifumi; null (UsOnly)
YAMAGUCHI, Takao; null (UsOnly)
TAKITA, Masaki; null (UsOnly)
KANEKA CORPORATION[JP/JP]; 2-4, Nakanoshima 3-chome, Kita-ku, Osaka-shi, Osaka 5308288, JP (AllExceptUS)
Inventors: YANAGITA, Yoshifumi; null
YAMAGUCHI, Takao; null
TAKITA, Masaki; null
Agent: PATENT CORPORATE BODY ARCO PATENT OFFICE; 3rd Floor, Bo-eki Bldg., 123-1, Higashimachi, Chuo-ku, Kobe-shi, Hyogo 6500031, JP
Priority Data:
2009-08258230.03.2009JP
Title (EN) METHOD FOR COLLECTING POLYHYDROXYALKANOATE
(FR) PROCÉDÉ DE RÉCUPÉRATION DE POLYHYDROXYALCANOATE
(JA) ポリヒドロキシアルカノエートの回収方法
Abstract:
(EN) A high-purity PHA (polyhydroxyalkanoate) is separated from PHA-containing microbial cells by a commercially suitable, simple and low-cost method with a reduced number of processes, without causing a significant decrease in the molecular weight. Specifically disclosed is a method for collecting a polyhydroxyalkanoate, which comprises: (a) a step wherein an enzymatic treatment is performed by adding an enzyme and an alkali and/or a surfactant into an aqueous suspension of polyhydroxyalkanoate-containing microbial cells, thereby obtaining an enzymatic treatment liquid; (b) a step wherein the enzymatic treatment liquid is subjected to a physical crushing process under basic conditions in the presence of a surfactant, so that the cells are crushed and cell substances other than polyhydroxyalkanoate in the cells are solubilized or emulsified, thereby obtaining a polyhydroxyalkanoate suspension; and (c) a step wherein the polyhydroxyalkanoate is separated from the polyhydroxyalkanoate suspension.
(FR) Un PHA (polyhydroxyalcanoate) de pureté élevée est séparé à partir de cellules microbiennes contenant du PHA par une méthode commerciale simple et économique qui comporte un nombre réduit de procédés, n'entraînant pas de diminution significative du poids moléculaire. Le procédé de récupération de polyhydroxyalcanoate spécifiquement décrit dans la présente invention comprend : (a) une étape de traitement enzymatique consistant à ajouter une enzyme et un alcali et/ou un tensioactif à une suspension aqueuse de cellules microbiennes contenant un polyhydroxyalcanoate, pour obtenir ainsi un liquide de traitement enzymatique ; (b) une étape de soumission du liquide de traitement enzymatique à un procédé de broyage physique dans des conditions basiques en présence d'un tensioactif, de façon que les cellules soient broyées et que les substances cellulaires autres que le polyhydroxyalcanoate dans les cellules soient solubilisées ou émulsifiées, pour obtenir ainsi une suspension de polyhydroxyalcanoate ; et (c) une étape de séparation du polyhydroxyalcanoate à partir de la suspension de polyhydroxyalcanoate.
(JA)  工業生産に適した簡便且つ安価な方法により、少ない工程数で、大幅な分子量低下を起こすことなく、PHA含有微生物細胞から高純度のPHAを分離する。ポリヒドロキシアルカノエート含有微生物細胞の水性懸濁液に、酵素、並びに、アルカリ及び/又は界面活性剤を添加して酵素処理を行い、酵素処理液を得る工程(a)、前記酵素処理液に対し、塩基性条件下、界面活性剤の存在下で物理的破砕処理を行ない、前記細胞を破砕すると共に、前記細胞中のポリヒドロキシアルカノエート以外の細胞物質を可溶化又は乳化させて、ポリヒドロキシアルカノエート懸濁液を得る工程(b)、前記ポリヒドロキシアルカノエート懸濁液からポリヒドロキシアルカノエートを分離する工程(c)、を行う。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (EPO) (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)