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1. (WO2010116595) METHOD FOR DETERMINING BIOPOLYMER USING NANOPORE, AND SYSTEM AND KIT THEREFOR
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2010/116595 International Application No.: PCT/JP2010/001352
Publication Date: 14.10.2010 International Filing Date: 01.03.2010
IPC:
G01N 33/50 (2006.01) ,C12Q 1/68 (2006.01) ,G01N 21/64 (2006.01) ,G01N 21/78 (2006.01) ,G01N 27/00 (2006.01) ,G01N 27/447 (2006.01)
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21
Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible, or ultra-violet light
62
Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63
optically excited
64
Fluorescence; Phosphorescence
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21
Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible, or ultra-violet light
75
Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
77
by observing the effect on a chemical indicator
78
producing a change of colour
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
27
Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
27
Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
26
by investigating electrochemical variables; by using electrolysis or electrophoresis
416
Systems
447
using electrophoresis
Applicants:
坂井 友幸 SAKAI, Tomoyuki [JP/JP]; JP (UsOnly)
藤田 毅 FUJITA, Takeshi [JP/JP]; JP (UsOnly)
株式会社日立ハイテクノロジーズ HITACHI HIGH-TECHNOLOGIES CORPORATION [JP/JP]; 東京都港区西新橋一丁目24番14号 24-14, Nishi Shimbashi 1-chome, Minato-ku, Tokyo 1058717, JP (AllExceptUS)
Inventors:
坂井 友幸 SAKAI, Tomoyuki; JP
藤田 毅 FUJITA, Takeshi; JP
Agent:
井上 学 INOUE, Manabu; 東京都千代田区丸の内一丁目6番1号 株式会社日立製作所内 c/o HITACHI, LTD. 6-1, Marunouchi 1-chome Chiyoda-ku, Tokyo 1008220, JP
Priority Data:
2009-08086730.03.2009JP
Title (EN) METHOD FOR DETERMINING BIOPOLYMER USING NANOPORE, AND SYSTEM AND KIT THEREFOR
(FR) PROCÉDÉ DE DÉTERMINATION DE BIOPOLYMÈRE À L'AIDE D'UN NANOPORE, ET SYSTÈME ET COFFRET POUR CELUI-CI
(JA) ナノポアを用いたバイオポリマー決定方法、システム、及びキット
Abstract:
(EN) Although analysis can be very quickly conducted at a low cost by a method for measuring a biopolymer using a nanopore, the accuracy of distinguishing the individual monopolymers constituting the biopolymer is low. To both ends of a biopolymer through a nanopore, molecules which are larger than the nanopore are attached and then the biopolymer is reciprocated by an external force to thereby perform repeated measurements.
(FR) Bien qu'une analyse puisse être conduite extrêmement rapidement à un coût faible par un procédé de mesure d'un biopolymère à l'aide d'un nanopore, la précision de la distinction des monopolymères individuels constituant le biopolymère est faible. Selon l'invention, des molécules qui sont plus grandes qu'un nanopore, sont attachées aux deux extrémités d'un biopolymère à travers le nanopore, puis le biopolymère est animé d'un mouvement de va-et-vient par une force externe pour effectuer ainsi des mesures répétées.
(JA) ナノポアを用いたバイオポリマーの計測方法は,非常に高速,安価分析であるが,バイオポリマーを構成するモノポリマ一つずつを判別する精度が低かった。 バイオポリマーのナノポアを介した両端にナノポアよりも大きな分子を結合し,外力によって前記バイオポリマーを往復運動させ,繰返し計測を行う。
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (EPO) (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)