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1. (WO2010116020) STRAINS OF S.CEREVISIAE, CAPABLE OF GROWING IN MEDIA WITH MELIBIOSE, STACHYOSE AND RAFFINOSE
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2010/116020    International Application No.:    PCT/ES2010/070219
Publication Date: 14.10.2010 International Filing Date: 08.04.2010
Chapter 2 Demand Filed:    08.02.2011    
IPC:
C12N 9/40 (2006.01), C12N 9/42 (2006.01), C12N 15/81 (2006.01)
Applicants: UNIVERSIDADE DA CORUÑA [ES/ES]; Maestranza, 9 E-15001 A Coruña (ES) (For All Designated States Except US).
BECERRA FERNÁNDEZ, Manuel [ES/ES]; (ES) (For US Only).
CERDÁN VILLANUEVA, María Esperanza [ES/ES]; (ES) (For US Only).
FERNÁNDEZ LEIRO, Rafael [ES/ES]; (ES) (For US Only).
GONZÁLEZ SISO, María Isabel [ES/ES]; (ES) (For US Only).
PEREIRA RODRÍGUEZ, Ángel [ES/ES]; (ES) (For US Only)
Inventors: BECERRA FERNÁNDEZ, Manuel; (ES).
CERDÁN VILLANUEVA, María Esperanza; (ES).
FERNÁNDEZ LEIRO, Rafael; (ES).
GONZÁLEZ SISO, María Isabel; (ES).
PEREIRA RODRÍGUEZ, Ángel; (ES)
Agent: ARIAS SANZ, Juan; ABG Patentes, S.L. Avenida de Burgos, 16D Edificio Euromor E-28036 Madrid (ES).
ALCÓNADA RODRÍGUEZ, Agustiπ; ABG Patentee, S-L Avda- de Burgos, 16D - Edificto Euromor E-28036 Madrid (ES)
Priority Data:
P200900961 08.04.2009 ES
Title (EN) STRAINS OF S.CEREVISIAE, CAPABLE OF GROWING IN MEDIA WITH MELIBIOSE, STACHYOSE AND RAFFINOSE
(ES) CEPAS DE S.CEREVISIAE CAPACES DE CRECER EN MEDIOS CON MELIBIOSA, ESTAQUIOSA Y RAFINOSA
(FR) SOUCHES DE S. CEREVISIAE POUVANT SE DÉVELOPPER DANS DES MILIEUX RICHES EN MÉLIBIOSE, STACHYOSE ET RAFFINOSE
Abstract: front page image
(EN)The invention relates to strains of S. cerevisae that are capable of producing @-galactosidase under the control of a heterologous promoter regulated by means of repression by glucose, specifically ADH2. For the purpose of improved secretion, a signal sequence is fused to the gene of the @-galactosidase, specifically yeast α-factor. Said strains can be used for the production of @-galactosidase, biomass and ethanol from a medium rich in raffinose, melibiose and/or stachyose.
(ES)La invención se relaciona con cepas de S. cerevisae que son capaces de producir @-galactosidasa bajo el control de un promotor heterólogo regulado mediante represión por glucosa, en concreto ADH2. Para su mejor secreción, el gen de la @-galactosidasa tiene fusionada una secuencia señal, en concreto el factor α de levadura. Dichas cepas son útiles para la producción de @-galactosidasa, de biomasa y de etanol, a partir de un medio rico en rafinosa, melibiosa y/o estaquinosa.
(FR)L'invention concerne des souches de S. cerevisiae pouvant produire de l'alpha-galactosidase sous le contrôle d'un promoteur hétérologue régulé par répression par glucose, soit ADH2. En vue d'une sécrétion améliorée, le gène de l'alpha-galactosidase comprend une séquence signal fusionnée, soit le facteur alpha de levure. Lesdites souches sont utiles pour la production d'alpha-galactosidase, de biomasse et d'éthanol, à partir d'un milieu riche en raffinose, mélibiose et/ou stachyose.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: Spanish (ES)
Filing Language: Spanish (ES)