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1. (WO2010114772) FLUORESCENCE CUED RAMAN IDENTIFICATION OF VIABLE ORGANISMS
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2010/114772 International Application No.: PCT/US2010/028869
Publication Date: 07.10.2010 International Filing Date: 26.03.2010
Chapter 2 Demand Filed: 03.02.2011
IPC:
C12Q 1/02 (2006.01) ,C12Q 1/04 (2006.01) ,G01N 33/50 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
02
involving viable micro-organisms
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
02
involving viable micro-organisms
04
Determining presence or kind of micro-organism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
Applicants: CARPENTER, Jeffrey, P.[US/US]; US (UsOnly)
BATTELLE MEMORIAL INSTITUTE[US/US]; A Non-profit Corporation Of The State Of Ohio 505 King Avenue Columbus, OH 43201, US (AllExceptUS)
Inventors: CARPENTER, Jeffrey, P.; US
Agent: LEES, Thomas, E.; Thomas E. Lees, LLC 70 Rhoads Center Drive, Suite B Dayton, Ohio 45458, US
Priority Data:
61/166,39903.04.2009US
Title (EN) FLUORESCENCE CUED RAMAN IDENTIFICATION OF VIABLE ORGANISMS
(FR) COLLECTE ET DÉTECTION BIOLOGIQUES ET CHIMIQUES
Abstract:
(EN) A fluorescence-cued Raman identification system comprises a collection subsystem to collect samples, a reagent treatment subsystem to treat collected samples and a fluorescence imaging subsystem that automatically takes at least one image of the collected sample. The subsystems further include a Raman spectroscopy subsystem that measures the spectrum of viable organisms located from at least one collected image, a visible imaging microscope subsystem that provides a visual image of the particle analyzed by the Raman spectroscopy subsystem and a processor configured to perform image processing to locate viable organisms within the sample, which are targeted by the Raman spectroscopy subsystem. The processor further analyzes the spectrum recorded by the Raman spectroscopy subsystem to make a preliminary identification of the targeted organisms, which can be verified by an operator using the visible imaging microscope subsystem.
(FR) La présente invention concerne un système d'identification Raman basé sur la fluorescence qui comprend un sous-système de collecte destiné à colleter des échantillons, un sous-système de traitement par réactif destiné à traiter les échantillons collectés et un sous-système d'imagerie de fluorescence qui prend automatiquement au moins une image de l'échantillon collecté. Les sous-systèmes comprennent en outre un sous-système de spectroscopie Raman qui mesure le spectre des organismes viables localisés sur au moins une image collectée, un sous-système de microscope d'imagerie visible qui fournit une image visuelle de la particule analysée par le sous-système de spectroscopie Raman et un processeur configuré pour réaliser un traitement d'image et localiser les organismes viables à l'intérieur de l'échantillon, qui sont ciblés par le sous-système de spectroscopie Raman. Le processeur analyse en outre le spectre enregistré par le sous-système de spectroscopie Raman pour faire une identification préliminaire des organismes ciblés, ce qui peut être vérifié par un opérateur utilisant le sous-système de microscope d'imagerie visible.
front page image
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (EPO) (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)